Mexneurin is a novel precursor of peptides in the central nervous system of rodents.
Mexneurin is a novel precursor of peptides in the central nervous system of rodents.
复制标题
Mexneurin 是啮齿动物中枢神经系统中肽的新型前体。
作者：
Matus-Ortega,MauraE;LeffGelman,Philippe;Calva-Nieves,JuanC;Flores-Zamora,Anabel;Salazar-Juárez,Alberto;Torner-Aguilar,Carlos-Alejandro;Gamba,Gerardo;DeLosHeros,Paola;Peng,Bonnie;Pintar,JohnE;Gompf,HeinrichS;Allen,CharlesN;A
内吗啡肽 (EM) 已被提议作为 μ-阿片受体的内源性配体激动剂；然而，尚未鉴定出 EM 的前肽前体蛋白。在这里，为了识别推测的 EM 前体，我们设计了一种免疫筛选试验，使用针对全小鼠脑 cDNA 文库中合成 EM 的特异性亲和纯化兔抗血清。按照这种方法，我们鉴定了编码蛋白质前体的 DNA 序列，我们将其命名为 proMexneurin，它包含三种不同的肽序列：Mexneurin-1（一种类似 EM 的肽）、Mexneurin-2 和 Mexneurin-3（一种似乎与 EM 无关的肽）。 RT-PCR 分析和原位杂交揭示了 proMexneurin mRNA 在整个小鼠大脑中的广泛分布。 Mexneurin-1 和 Mexneurin-3 肽均在小鼠 CNS 中显示出生物活性。
Endomorphins (EMs) have been proposed as the endogenous ligand agonists of the μ‐opioid receptor; however, no propeptide precursor protein for EMs has been identified. Here, to identify the presumed precursor of EMs, we designed an immunoscreening assay using specific affinity‐purified rabbit antisera raised against synthetic EMs in a whole‐mouse brain cDNA library. Following this approach, we identify a DNA sequence encoding a protein precursor, which we name proMexneurin, that contains three different peptide sequences: Mexneurin‐1 (an EM‐like peptide), Mexneurin‐2, and Mexneurin‐3, a peptide which appears to be unrelated to EMs. RT‐PCR analysis andin situhybridization reveal a widespread distribution of proMexneurin mRNA throughout the mouse brain. Both Mexneurin‐1 and Mexneurin‐3 peptides display biological activities in the mouse CNS.