Labedella endophytica sp.nov., a novel endophytic actinobacterium isolated from stem of Anabasis elatior (C.A.Mey.)Schischk.
Labedella endophytica sp.nov., a novel endophytic actinobacterium isolated from stem of Anabasis elatior (C.A.Mey.)Schischk.
复制标题
内生标签菌 sp.
DOI：
10.1007/s10482-014-0307-0
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Wen-Jun Li
中科院分区：
文献类型：
--
作者：
Hong-Fei Wang;Yong-Guang Zhang;Juan Cheng;Wael N.Hozzein;Li Li;Ji-Yue Chen;Jian-Wei Guo;Yuan-Ming Zhang;Wen-Jun Li
从盐生植物高大假木贼（Anabasiselatior）表面灭菌的茎中分离到一株革兰氏染色阳性、黄色、需氧、非运动、过氧化氢酶阳性和氧化酶阴性的内生放线菌，命名为EGI 6500705（T）。A.梅。）采自中国西北部新疆省乌鲁木齐市。该生物体具有作为诊断细胞壁二氨基酸的鸟氨酸。主要脂肪酸为anteiso-C15：0、anteiso-C17：0和iso-C16：0。主要的甲基萘醌是MK-9、MK-10和MK-11。菌株EGI 6500705（T）的DNA G + C含量为69.2mol%。系统发育分析表明，EGI 6500705（T）与Labedella gwakjiensis KCTC 19176（T）的同源性高达99.0%，属于Labedella属。菌株EGI 6500705（T）与L. gwakjiensisKCTC 19176（T）为27.4%。根据表型特征、化学分类学和系统发育分析，EGI 6500705（T）菌株代表Labedella属的一个新种，命名为Labedella endophytica sp. nov.。模式菌株为EGI 6500705（T）（=KCTC 29494（T）= CPCC 203961（T）= JCM 30092（T））。
A Gram-stain positive, yellow-coloured, aerobic, non-motile, catalase-positive and oxidase-negative, endophytic actinobacterium, designated strain EGI 6500705(T), was isolated from the surface-sterilized stem of a halophyte Anabasis elatior (C. A. Mey.) Schischk collected from Urumqi, Xinjiang province, north-west China. The organism had ornithine as the diagnostic cell-wall diamino acid. The major fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C16:0. The predominant menaquinones were MK-9, MK-10 and MK-11. The DNA G + C content of strain EGI 6500705(T) was 69.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain EGI 6500705(T) is clearly affiliated with the genus Labedella and most closely related to Labedella gwakjiensis KCTC 19176(T), with 99.0 % sequence similarity. DNA-DNA relatedness between strain EGI 6500705(T) and L. gwakjiensis KCTC 19176(T) was 27.4 %. On the basis of phenophytic, chemotaxonomic and phylogenetic analysis, the strain EGI 6500705(T) represents a novel species of the genus Labedella, for which the name Labedella endophytica sp. nov. is proposed. The type strain is EGI 6500705(T) (=KCTC 29494(T) = CPCC 203961(T) = JCM 30092(T)).