THE ESSENTIAL ROLE OF CALCIUM ION IN POLLEN GERMINATION AND POLLEN TUBE GROWTH
THE ESSENTIAL ROLE OF CALCIUM ION IN POLLEN GERMINATION AND POLLEN TUBE GROWTH
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DOI：
10.1002/j.1537-2197.1963.tb06564.x
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发表时间：
1963-10
影响因子：
3
通讯作者：
J. Brewbaker;B. Kwack
中科院分区：
文献类型：
--
作者：
J. Brewbaker;B. Kwack
詹姆斯·布鲁贝克年轻的H。咔。（美国）夏威夷、檀香山）钙离子在花粉萌发和花粉管生长中的重要作用。美国日报机器人50（9）：859-865。伊卢斯1963.-花粉群体效应发生在花粉粒在体外生长的任何时候。小花粉群体在支持大花粉群体良好生长的条件下发芽和生长不良。花粉群体效应被许多植物组织的水提取物中获得的生长因子完全克服。该因子被证明是钙离子，并证实其作用在86种植物，代表39个科。其他离子（K+、Mg++、Na+）对钙的吸收或结合起支持作用。大多数花粉对钙的高需求量（300-5000 ppm，以Ca（NO3）2·4 H20计，最佳生长）和低钙含量可能共同给予钙在试管和原位花粉管生长中的控制作用。人们怀疑，这种作用的后果延伸到植物的自交不亲和性和奇怪的类型的逮捕管生长区分，例如，兰花。在各种花粉生长研究中证明其优点的培养基包括在蒸馏水中的10%蔗糖、100 ppm H3 BO 3、300 ppm Ca（NO3）2。4H20，200 ppm MgSO4。7H20
BREWBAKER, JAMES L., and BEYOUNG H. KWACK. (U. Hawaii, Honolulu.) The essential role of calcium ion in pollen germination and pollen tube growth. Amer. Jour. Bot. 50(9): 859-865. Illus. 1963.-A pollen population effect occurs whenever pollen grains are grown in vitro. Small pollen populations germinate and grow poorly if at all, under conditions which support excellent growth of large pollen populations. The pollen population effect is overcome completely by a growth factor obtained in water extracts of many plant tissues. This factor is shown to be the calcium ion, and its action confirmed in86 species representing 39plant families. Other ions (K+, Mg++, Na+) serve in supporting roles to the uptake or binding of calcium. The high requirement of calcium (300-5000 ppm, as Ca (NO3)2 4H20, for optimum growth) and low calcium content of most pollen may conspire to give calcium a governing role in the growth of pollen tubes both in vitro and in situ. It is suspected that ramifications ofthis role extend to the self-incompatibilities of plants and to the curious types of arrested tube growth distinguishing, for example, the orchids. A culture medium which proved its merit in a wide variety of pollen growth studies included, in distilled water, 10% sucrose, 100 ppm H3BO3, 300 ppm Ca (NO3)2. 4H20, 200 ppm MgSO4. 7H20