Challenging the putative structure of mannan in wheat (Triticum aestivum) endosperm.
Challenging the putative structure of mannan in wheat (Triticum aestivum) endosperm.
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DOI：
10.1016/j.carbpol.2019.115063
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发表时间：
2019-11
影响因子：
11.2
通讯作者：
Yves Verhertbruggen;X. Falourd;Marlayna Sterner;F. Guillon;C. Girousse;L. Foucat;S. Le Gall;A. Chateigner-Boutin;L. Saulnier
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文献类型：
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作者：
Yves Verhertbruggen;X. Falourd;Marlayna Sterner;F. Guillon;C. Girousse;L. Foucat;S. Le Gall;A. Chateigner-Boutin;L. Saulnier
在小麦胚乳中，甘露聚糖的记载很少。然而，这种半纤维素多糖可能在小麦籽粒发育中具有决定性作用，因为在拟南芥中，甘露聚糖含量减少的突变体显示出种子发育的改变。为了了解小麦中的甘露聚糖，我们测定了小麦胚乳中甘露糖的生化结构，其中甘露糖含量约为0.2%（以干重计）。我们开发了一种酶指纹法和分离富含甘露聚糖的级分来破译其精细结构。尽管人们普遍认为存在于草细胞壁中的甘露聚糖是葡甘露聚糖，但我们的数据表明，在小麦胚乳中，这种半纤维素仅由 1,4 连接的 D-甘露糖残基的短未取代链代表，并且是轻微乙酰化的。我们的研究提供了有关甘露聚糖与其他细胞壁成分相互作用的信息，并有助于加深对单子叶植物细胞壁结构和小麦胚乳中甘露聚糖合成的理解。
In wheat endosperm, mannan, is poorly documented. Nevertheless, this hemicellulosic polysaccharide might have a determinant role in wheat grain development since, in Arabidopsis thaliana, mutants with a reduced amount of mannan show an altered seed development. In order to gain knowledge about mannan in wheat, we have determined its biochemical structure in wheat endosperm where mannose content is about 0.2% (dry weight basis). We developed a method of enzymatic fingerprinting and isolated mannan-enriched fractions to decipher its fine structure. Although it is widely accepted that the class of mannan present in grass cell walls is glucomannan, our data indicate that, in wheat endosperm, this hemicellulose is only represented by short unsubstituted chains of 1,4 linked D-mannose residues and is slightly acetylated. Our study provides information regarding the interactions of mannan with other cell wall components and help to progress towards the understanding of monocot cell wall architecture and the mannan synthesis in wheat endosperm.