Novel circular RNA 2960 contributes to secondary damage of spinal cord injury by sponging miRNA-124
Novel circular RNA 2960 contributes to secondary damage of spinal cord injury by sponging miRNA-124
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DOI：
10.1002/cne.25030
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发表时间：
2020-11-11
影响因子：
2.5
通讯作者：
Liu, Zhongtao
中科院分区：
文献类型：
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作者：
Chen, Jun;Fu, Bin;Liu, Zhongtao
近年来的研究表明，环状RNA（circular RNA，circRNA）与多种人类疾病有关，但其在脊髓损伤（spinal cord injury，SCI）后继发性损伤中的作用尚不清楚。在本研究中，在SCI大鼠损伤后第7天的损伤组织中进行circRNA测序，并研究相关的分子机制。对SCI小鼠中表现出显著改变的表达的分子进行定量PCR验证。进行基因本体和京都基因和基因组百科全书分析以评估差异表达的circRNA。一种新的circRNA-2960在SCI组中表达最显著。它可以下调其靶分子miRNA-124，从而加剧炎症反应并诱导损伤部位的细胞凋亡。干扰circRNA-2960的表达促进了继发性SCI损伤的组织恢复。本研究结果可能为SCI继发性损伤的机制提供新的认识，并为SCI的诊断和治疗提供新的分子标志物。
Recent studies have shown that circular RNAs (circRNAs) are involved in many human diseases, but their roles in secondary damage after spinal cord injury (SCI) remain unclear. In the current study circRNA sequencing was performed in the damaged tissues of SCI rats on the seventh day after injury, and related molecular mechanisms were investigated. Quantitative PCR validations of molecules that exhibited significantly altered expression in SCI mice were performed. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were conducted to assess differentially expressed circRNAs. A novel circRNA-2960 was the most significantly upregulated in the SCI group. It could downregulate its target molecule miRNA-124, then exacerbate the inflammatory response and induce apoptosis at the lesion site. Disrupting circRNA-2960 expression promoted recovery of tissues affected by secondary SCI damage. The results of the present study may provide new insight into the mechanisms of secondary injury in SCI, and a new molecular marker for the diagnosis and treatment of SCI.