The importance of serum albumin and metabolic intermediates for capacitation of spermatozoa and fertilization of mouse eggs in vitro.
The importance of serum albumin and metabolic intermediates for capacitation of spermatozoa and fertilization of mouse eggs in vitro.
复制标题
血清白蛋白和代谢中间体对于精子获能和小鼠卵子体外受精的重要性。
DOI：
10.1530/jrf.0.0320193
复制
发表时间：
1973
期刊：
影响因子：
--
通讯作者：
M. Chang
中科院分区：
文献类型：
--
作者：
H. Miyamoto;M. Chang
总结。研究了小鼠附睾精子顶体反应和小鼠卵子在不含组织液的化学介质中的体外受精。在含有葡萄糖的改良Krebs-Ringer碳酸氢盐基本培养液中孵育5~7小时，约有8%~10%的活动精子失去顶体，但没有卵子穿透。在基础培养液中加入单一代谢中间体草酰乙酸钠或丙酮酸钠，可增加无顶体活动精子的比例(19~34%)和穿透卵子的比例(3~2~25~5%)。在含有不同种类血清白蛋白的基本培养液中孵育精子和卵子，可使无顶体的活动精子比例(50%~65%)和穿透精子比例(60%~86%)进一步增加。小鼠卵子体外获能和受精的最佳基础培养液为牛血清白蛋白+乳酸钠+丙酮酸钠。精子获能所需时间在该培养液中为1小时，在仅含血清白蛋白的培养液中为2小时。输卵管液和卵丘中存在的某些成分，可能类似于血清白蛋白和乳酸钠或丙酮酸钠，似乎有利于精子的获能和卵子的受精。结论：血清白蛋白、乳酸钠和丙酮酸钠可替代组织液在体外诱导小鼠精子获能和卵子受精。
Summary. The acrosome reaction of epididymal spermatozoa and fertilization in vitro of mouse eggs in chemically defined media without tissue fluid were investigated. About 8 to 10% of motile spermatozoa lost their acrosome but no eggs were penetrated when the spermatozoa and eggs were incubated in a basic medium (modified Krebs-Ringer bicarbonate solution containing glucose) for 5 to 7 hr. Addition of a single metabolic intermediate, such as sodium oxaloacetate or sodium pyruvate, to the basic medium increased the proportion of motile spermatozoa without an acrosome (19 to 34%) and the proportion of eggs penetrated (3\m=.\2 to 25\m=.\5%). Incubation of spermatozoa and eggs in the basic medium containing serum albumin of various species caused a further increase in the proportion of motile spermatozoa without an acrosome (50 to 65%) and in that of penetrated eggs (60\m=.\7 to 86%). The best medium for sperm capacitation and fertilization of mouse eggs in vitro, however, was the basic medium containing bovine serum albumin, sodium lactate and sodium pyruvate. The time required for sperm capacitation was 1 hr in this medium, and 2 hr in the medium containing only serum albumin. Certain components present in the oviducal fluid and in the cumulus egg clots, probably similar to serum albumin and sodium lactate or sodium pyruvate, appeared to be beneficial for the capacitation of spermatozoa and fertilization of eggs. It was concluded that serum albumin, sodium lactate and sodium pyruvate can be substituted for tissue fluid in the induction of capacitation of spermatozoa and fertilization of mouse eggs in vitro.