Effect of immunological adjuvants: GM-CSF (granulocyte-monocyte colony stimulating, factor) and IL-23 (interleukin-23) on immune responses generated against hepatitis C virus core DNA vaccine
Effect of immunological adjuvants: GM-CSF (granulocyte-monocyte colony stimulating, factor) and IL-23 (interleukin-23) on immune responses generated against hepatitis C virus core DNA vaccine
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DOI：
10.1016/j.cyto.2008.12.007
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发表时间：
2009-04-01
期刊：
影响因子：
3.8
通讯作者：
Azadmanesh, Kayhan
中科院分区：
文献类型：
--
作者：
Hartoonian, Christine;Ebtekar, Massoumeh;Azadmanesh, Kayhan
使用细胞因子作为佐剂已被证明是增强DNA疫苗诱导的免疫应答的有前途的方法。在这份报告中，我们研究了管理的细胞因子，以调节体液和细胞介导的免疫反应引起的HCV核心质粒DNA疫苗在Balb/c小鼠。我们的研究表明，HCV核心DNA疫苗已经能够在DNA引物-蛋白加强方案中诱导抗体和细胞免疫。GM-CSF（粒细胞-单核细胞集落刺激因子）被认为是一种同时显示Th 1和Th 2特征的细胞因子，在增强抗体和细胞介导的免疫中发挥着重要作用。在这种情况下，细胞免疫的诱导不像体液免疫那样显著。为了获得更强的细胞应答，该方案中还包括IL-23，一种属于IL-12家族的Th 1细胞因子。脾细胞增殖、脾细胞产生IFN-γ和特异性血清IgG 2a均显示细胞介导的免疫增强，而对抗体和体液免疫应答无任何可观察到的抑制作用。我们还检查了质粒IL-23给药的时间对所产生的T细胞应答表型的影响，在质粒GMCSF给药之前和之后，间隔3天。结果表明，与同时施用IL-23相比，Th 1/Th 2平衡没有任何变化。(C)2008爱思唯尔有限公司保留所有权利。
The use of cytokines as adjuvants has been shown to be a promising approach for enhancing DNA vaccine induced-immune responses. In this report, we investigate the administration of cytokines to modulate both humoral and cell-mediated immune responses elicited by an HCV-core plasmid DNA vaccine in Balb/c mice. Our studies indicate that the HCV-core DNA vaccine has been able to induce both antibody and cellular immunity in a DNA prime-protein boost regimen. GM-CSF (granulocyte-monocyte colony stimulating factor) which is considered to be a cytokine displaying both Th1 and Th2 characteristics, and plays an important role in augmenting antibody and cell-mediated immunity was also administered. The induction of cellular immunity was not as striking as humoral immunity in this case. To obtain a stronger cellular response, IL-23, a Th1 cytokine belonging to the IL-12 family, was also included in the regimen. Spleen cell proliferation, IFN-gamma production from spleen cells and specific serum IgG2a, all demonstrate the enhancement of cell-mediated immunity without any observable suppressive effect on antibody and humoral immune responses. We also examined the timing of plasmid IL-23 administration on the phenotype of the resultant T cell responses in a 3 day interval, before and after plasm id GMCSF administration. The results did not indicate any change in the Th1/Th2 balance as compared with simultaneous IL-23 administration. (C) 2008 Elsevier Ltd. All rights reserved.