Photoacoustic imaging of small organic molecule-based photoacoustic probe in subcutaneous tumor using P(VDF-TrFE) acoustic sensor

Photoacoustic imaging of small organic molecule-based photoacoustic probe in subcutaneous tumor using P(VDF-TrFE) acoustic sensor
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使用 P(VDF-TrFE) 声传感器对皮下肿瘤中基于有机小分子的光声探针进行光声成像

DOI：
10.1117/12.2081223
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发表时间：
2015
期刊：
Proceedings of SPIE
影响因子：
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通讯作者：
Takeshi Hirasawa,Shinpei Okawa,Mako Kamiya,Yasuteru Urano,Miya Ishihara
Takeshi Hirasawa,Shinpei Okawa,Mako Kamiya,Yasuteru Urano,Miya Ishihara
中科院分区：
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文献类型：
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作者：
Shohei Mori;Shinnosuke Hirata;Tadashi Yamaguchi;Hiroyuki Hachiya;Takeshi Hirasawa,Shinpei Okawa,Mako Kamiya,Yasuteru Urano,Miya Ishihara

文献摘要

相似文献

研制了用于多光谱光声成像（MS-PAI）的P（VDF-TrFE）传感器，该传感器在2.9 ~ 19.6 MHz频率范围内具有均匀灵敏度。本课题组合成的基于有机分子的PA小探针在530 nm处吸收最大，用作造影剂。设计了低量子产率的PA探针。因此，PA探头有效地将吸收的光能转换为PA信号。将探针注射到小鼠皮下肿瘤中。然后用P（VDF-TrFE）传感器对皮下肿瘤进行活体成像。MS-PAI成功地从血红蛋白等内源性光吸收剂产生的背景信号中区分出探针信号。评估了P（VDF-TrFE）传感器的探头可探测性，并与钛酸铅锆（PZT）传感器的探头可探测性进行了比较。P（VDF-TrFE）传感器比中心频率为20 MHz的PZT传感器对肿瘤的成像更清晰，特别是当探针在低浓度的肿瘤中积累时。这是因为低浓度的探头产生的PA信号频率较低。采用P（VDF-TrFE）传感器的MS-PAI可以检测频率范围较宽的PA信号，能够对探头的各种分布进行成像，优于采用PZT传感器检测频率范围较窄的PA信号。
The P(VDF-TrFE) sensor which had uniform sensitivity in a frequency range of 2.9 – 19.6 MHz was developed for multispectral photoacoustic imaging (MS-PAI). A small organic molecule-based PA probe synthesized by our group had the absorption maximum at 530 nm and was used as a contrast agent. The PA probe was designed to have low quantum yield. Therefore, the PA probe efficiently converted absorbed optical energies to PA signals. The probe was injected in subcutaneous tumor of mice. Then, the subcutaneous tumor was imaged in vivo by using P(VDF-TrFE) sensor. MS-PAI successfully discriminated the probe signals from background signals produced from endogenous optical absorbers such as hemoglobin. The probe detectability of the P(VDF-TrFE) sensor was evaluated and then compared with that of lead zirconium titanate (PZT) sensors. The P(VDF-TrFE) sensor imaged the tumor more clearly than the PZT sensor with central frequency of 20 MHz, especially when the probe was accumulated in the tumor with low concentration. That was because the low-concentrated probe generated PA signals with low frequency. MS-PAI using P(VDF-TrFE) sensor which can detect PA signals with wide range of frequency is able to image various distribution of the probe and is superior to that using PZT sensor which detects PA signals with narrow frequency range.