(-)-Epigallocatechin gallate-loaded polycaprolactone scaffolds fabricated using a 3D integrated moulding method alleviate immune stress and induce neurogenesis
(-)-Epigallocatechin gallate-loaded polycaprolactone scaffolds fabricated using a 3D integrated moulding method alleviate immune stress and induce neurogenesis
复制标题
使用3D集成成型方法制造的(-)-表没食子儿茶素没食子酸酯负载的聚己内酯支架可减轻免疫应激并诱导神经发生
DOI：
10.1111/cpr.12730
复制
发表时间：
2019-11-20
影响因子：
8.5
通讯作者：
Ouyang, Yuanming
中科院分区：
文献类型：
--
作者：
Qian, Yun;Yao, Zhixiao;Ouyang, Yuanming
目的在周围神经病变中，神经和肌肉变性的潜在机制包括纤维化组织中的慢性炎症和氧化应激。 (-)-表没食子儿茶素没食子酸酯 (EGCG) 是绿茶中的主要活性成分，可以清除自由基氧并减轻炎症。类固醇注射等保守治疗只能治疗早期、无症状的周围神经病变。相比之下，神经松解术和神经导管植入对于治疗晚期疾病有效。材料和方法采用一体成型方法制造了负载EGCG的聚己内酯（PCL）多孔支架。我们评估了体外在不同支架上培养的大鼠雪旺细胞（RSC）和大鼠骨骼肌细胞（RSMC）的增殖、氧化和炎症活性。在大鼠辐射损伤模型中，我们评估了再生坐骨神经和腓肠肌的形态、电生理和功能表现，以及氧化应激和炎症状态。结果RSCs和RSMCs在EGCG/PCL支架中表现出更高的增殖、抗氧化和抗炎状态。体内研究表明，EGCG/PCL组的神经和肌肉恢复得到改善，神经髓鞘形成和肌纤维增殖增加，巨噬细胞浸润、脂质过氧化、炎症和氧化应激指标减少。结论 EGCG修饰的PCL多孔神经支架减轻了细胞氧化应激，修复了大鼠周围神经和肌肉结构。它可以减轻体内氧化应激和炎症，并可能为未来的周围神经修复提供进一步的见解。
ObjectivesIn peripheral neuropathy, the underlying mechanisms of nerve and muscle degeneration include chronic inflammation and oxidative stress in fibrotic tissues. (-)-Epigallocatechin gallate (EGCG) is a major, active component in green tea and may scavenge free radical oxygen and attenuate inflammation. Conservative treatments such as steroid injection only deal with early, asymptomatic, peripheral neuropathy. In contrast, neurolysis and nerve conduit implantation work effectively for treating advanced stages.Materials and methodsAn EGCG-loaded polycaprolactone (PCL) porous scaffold was fabricated using an integrated moulding method. We evaluated proliferative, oxidative and inflammatory activity of rat Schwann cells (RSCs) and rat skeletal muscle cells (RSMCs) cultured on different scaffolds in vitro. In a rat radiation injury model, we assessed the morphological, electrophysiological and functional performance of regenerated sciatic nerves and gastrocnemius muscles, as well as oxidative stress and inflammation state.ResultsRSCs and RSMCs exhibited higher proliferative, anti-oxidant and anti-inflammatory states in an EGCG/PCL scaffold. In vivo studies showed improved nerve and muscle recovery in the EGCG/PCL group, with increased nerve myelination and muscle fibre proliferation and reduced macrophage infiltration, lipid peroxidation, inflammation and oxidative stress indicators.ConclusionsThe EGCG-modified PCL porous nerve scaffold alleviates cellular oxidative stress and repairs peripheral nerve and muscle structure in rats. It attenuates oxidative stress and inflammation in vivo and may provide further insights into peripheral nerve repair in the future.