High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper.
High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper.
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DOI：
10.1016/j.biortech.2013.01.084
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发表时间：
2013-04
影响因子：
11.4
通讯作者：
Waldron KW
中科院分区：
文献类型：
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作者：
Elliston A;Collins SR;Wilson DR;Roberts IN;Waldron KW
在SSSF中批量添加废纸会产生高达11.6%（v/v）的乙醇。·低的总酶载量（3.7 FPU/g底物）。·高累积底物负载（65% w/v）。高乙醇浓度将提高蒸馏效率。第二代乙醇生产的基本目标是将乙醇浓度提高到10%（v/v）或更高，以优化蒸馏成本。半同时糖化和发酵（SSSF）以小的中试规模（5L）进行，利用固体切碎的复印纸基质的分批补料添加。以16 FPU/g底物和30 U/g β-葡糖苷酶早期添加Accellerase® 1500，然后仅分批添加底物，允许实现低的最终等效酶浓度（3.7 FPU/g底物），同时保持消化。批次添加导致累积底物浓度相当于65%（w/v）。这反过来又导致产生高浓度的乙醇（11.6% v/v）。该策略的成功依赖于生物反应器根据需要进行高剪切混合的能力。对底物添加时间和数量的进一步研究可能会导致总收率从65.5%进一步提高。
► Batch addition of paper waste in SSSF results in up to 11.6% (v/v) ethanol. ► Low overall enzyme loadings (3.7 FPU/g substrate). ► High cumulative substrate loadings (65% w/v). ► High ethanol concentrations will improve distillation efficiencies. A fundamental goal of second generation ethanol production is to increase the ethanol concentration to 10% (v/v) or more to optimise distillation costs. Semi simultaneous saccharification and fermentations (SSSF) were conducted at small pilot scale (5 L) utilising fed-batch additions of solid shredded copier paper substrate. Early addition of Accellerase® 1500 at 16 FPU/g substrate and 30 U/g β-glucosidase followed by substrate only batch addition allowed low final equivalent enzyme concentrations to be achieved (3.7 FPU/g substrate) whilst maintaining digestion. Batch addition resulted in a cumulative substrate concentration equivalent to 65% (w/v). This in turn resulted in the production of high concentrations of ethanol (11.6% v/v). The success of this strategy relied on the capacity of the bioreactor to perform high shear mixing as required. Further research into the timing and number of substrate additions could lead to further improvement in overall yields from the 65.5% attained.