Comparison of immunoreactive score, HER2/neu score and H score for the immunohistochemical evaluation of somatostatin receptors in bronchopulmonary neuroendocrine neoplasms
Comparison of immunoreactive score, HER2/neu score and H score for the immunohistochemical evaluation of somatostatin receptors in bronchopulmonary neuroendocrine neoplasms
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DOI：
10.1111/his.12662
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发表时间：
2015-09-01
期刊：
影响因子：
6.4
通讯作者：
Lupp, Amelie
中科院分区：
文献类型：
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作者：
Specht, Elisa;Kaemmerer, Daniel;Lupp, Amelie
目的随着越来越多的生长抑素受体(SSTR)靶向类似物和放射性多肽用于神经内分泌肿瘤(NEN)的诊断和治疗，对SSTR亚型状态的评估越来越具有预测价值。在病理学中，SSTR蛋白水平的常规检测是通过免疫组织化学(IHC)进行的；然而，缺乏标准化的评估系统仍然存在。因此，本研究比较了三种常用的半定量评分系统[免疫反应评分(IRS)、人表皮生长因子受体2(HER2)/neu评分、H评分]在NEN中的应用。方法与结果采用免疫组化和定量逆转录聚合酶链式反应(qRT-PCR)检测90例肺癌患者240例石蜡包埋肿瘤标本中SSTR1、2A、3、4和5的表达。在这两种方法中，SSTR1、2A和5是最常见的表达亚型。对于所有SSTR亚型，所有三个分数彼此之间以及与qRT-PCR数据都有很好的相关性。结论由于迫切需要一个统一的用于SSTR分析的IHC评分系统，以优化NEN的治疗效果，根据我们的结果，IRS评分似乎是最合适的。具有足够的精确度和较高的实用性。
AimsDue to the growing number of somatostatin receptor (SSTR) targeting analogues and radiopeptides used for the diagnosis and therapy of neuroendocrine neoplasms (NEN), the assessment of SSTR subtype status has increasingly gained predictive value. In pathology, the SSTR protein levels are detected routinely by immunohistochemistry (IHC); however, a lack of a standardized evaluation system persists. Thus, in the present investigation, three well-established semi-quantitative scoring systems [immunoreactive score (IRS), human epidermal growth factor receptor 2 (HER2)/neu score, H score] used commonly for SSTR-IHC evaluation in NEN were compared.Methods and resultsA total of 240 formalin-fixed, paraffin-embedded tumour samples from 90 patients with bronchopulmonary NEN were examined by IHC and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for SSTR1, 2A, 3, 4 and 5 expression. Using both methods, SSTR1, 2A and 5 were the most frequently expressed subtypes. For all SSTR subtypes, all three scores correlated well with each other and with qRT-PCR data. However, the IRS was the most meaningful score with the best correlation to mRNA levels.ConclusionsBecause a unified IHC scoring system for SSTR analysis is needed urgently to optimize the theranostics of NEN, among the scores tested, the IRS seems to be the most suitable according to our results. It provides sufficient accuracy combined with high practicability.