Water-Borne Nanocoating for Rapid Inactivation of SARS-CoV-2 and Other Viruses
Water-Borne Nanocoating for Rapid Inactivation of SARS-CoV-2 and Other Viruses
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DOI：
10.1021/acsnano.1c05075
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发表时间：
2021-08-23
期刊：
影响因子：
17.1
通讯作者：
Monteiro, Michael J.
中科院分区：
文献类型：
--
作者：
Bobrin, Valentin A.;Chen, Sung-Po;Monteiro, Michael J.
冠状病毒变种的增加导致了这种疾病在全球范围内的激增。病毒粒子膜表面的刺突蛋白的突变不仅允许更大的传播，而且还引起了对疫苗有效性的担忧。防止SARS-CoV-2及其变种和其他病毒通过空中或表面传播在人与人之间传播需要有效地灭活病毒。在这里，我们报告了一种水性喷涂层，用于完全灭活病毒颗粒并降解它们的RNA。我们的纳米蠕虫有效地结合，并通过随后的大规模纳米构象变化，打破病毒膜，随后结合并降解其RNA。我们的涂层完全灭活了SARS-CoV-2(VIC01)和一个进化的令人担忧的SARS-CoV-2变种(B.1.1.7(阿尔法))，甲型流感，以及一种表达甲型流感病毒附着糖蛋白血凝素的替代衣壳假病毒。纳米虫上的多聚半乳糖功能针对SARS-CoV-2病毒表面刺激性糖蛋白上保守的S2亚基进行更强的结合，以及额外的胍基团催化其RNA基因组的降解。用我们的纳米虫覆盖医用口罩，可以完全灭活VIC01和B.1.1.7，为SARS-CoV-2及其变种提供了强有力的控制措施。进一步观察到甲型流感病毒和AAV-HA衣壳假病毒的灭活，当使用纳米虫系统时，提供了广泛的病毒灭活。这里描述的技术代表了一种环境友好型涂层，具有拟议的纳米机械机制，可以灭活包膜病毒和衣壳病毒。这种功能性纳米虫可以很容易地进行修改，以在未来的大流行中针对病毒，并与大规模制造工艺兼容。
The rise in coronavirus variants has resulted in surges of the disease across the globe. The mutations in the spike protein on the surface of the virion membrane not only allow for greater transmission but also raise concerns about vaccine effectiveness. Preventing the spread of SARS-CoV-2, its variants, and other viruses from person to person via airborne or surface transmission requires effective inactivation of the virus. Here, we report a water-borne spray-on coating for the complete inactivation of viral particles and degradation of their RNA. Our nanoworms efficiently bind and, through subsequent large nanoscale conformational changes, rupture the viral membrane and subsequently bind and degrade its RNA. Our coating completely inactivated SARS-CoV-2 (VIC01) and an evolved SARS-CoV-2 variant of concern (B.1.1.7 (alpha)), influenza A, and a surrogate capsid pseudovirus expressing the influenza A virus attachment glycoprotein, hemagglutinin. The polygalactose functionality on the nanoworms targets the conserved S2 subunit on the SARS-CoV-2 virion surface spike glycoprotein for stronger binding, and the additional attachment of guanidine groups catalyze the degradation of its RNA genome. Coating surgical masks with our nanoworms resulted in complete inactivation of VIC01 and B.1.1.7, providing a powerful control measure for SARS-CoV-2 and its variants. Inactivation was further observed for the influenza A and an AAV-HA capsid pseudovirus, providing broad viral inactivation when using the nanoworm system. The technology described here represents an environmentally friendly coating with a proposed nanomechanical mechanism for inactivation of both enveloped and capsid viruses. The functional nanoworms can be easily modified to target viruses in future pandemics, and is compatible with large scale manufacturing processes.