Complementary DNA cloning and sequencing of rat ovarian basic fibroblast growth factor and tissue distribution study of its mRNA.
Complementary DNA cloning and sequencing of rat ovarian basic fibroblast growth factor and tissue distribution study of its mRNA.
复制标题
大鼠卵巢碱性成纤维细胞生长因子的互补DNA克隆和测序及其mRNA的组织分布研究。
DOI：
10.1016/s0006-291x(88)80041-x
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发表时间：
1988
影响因子：
3.1
通讯作者：
Ling,N
中科院分区：
文献类型：
--
作者：
Shimasaki,S;Emoto,N;Koba,A;Mercado,M;Shibata,F;Cooksey,K;Baird,A;Ling,N
从妊娠母马血清促性腺激素（PMSG）刺激大鼠卵巢cDNA文库制备的106个独立克隆中分离到3个编码大鼠碱性成纤维细胞生长因子（FGF）的cDNA克隆。其中一个cDNA克隆包含基本FGF的完整编码序列。另外两个具有编码大鼠碱性FGF羧基末端61个氨基酸的序列、推测的上游内含子序列和一个3 ' -非编码区。编码大鼠碱性FGF的cdna预测了一个由154个氨基酸残基组成的分子，比人类和牛碱性FGF短一个氨基酸。除此之外，大鼠和人/牛的序列之间只有5个保守的氨基酸替换。来自大脑皮层和下丘脑的Poly A+RNA显示一个6.0 kb的条带，通过Northern分析与克隆的cDNA探针杂交。基本FGF mRNA在肾上腺、脾、心、肺、肾、肝、胃、小肠、大肠、睾丸和卵巢中低于检测极限的观察结果支持了这样一种观点，即在这些组织中发现的高水平蛋白质是由于有丝分裂原储存在细胞外基质中，而不是连续的基因表达。在没有血管生成或细胞增殖的组织（下丘脑和大脑皮层）中，mRNA丰度的意义尚不清楚，但强调了基础FGF潜在的神经营养功能。
Three cDNA clones encoding rat basic fibroblast growth factor (FGF) were isolated from 106independent clones prepared from a pregnant mare serum gonadotropin (PMSG)-stimulated rat ovarian cDNA library. One of the cDNA clones contained the entire coding sequence for basic FGF. The other two possessed the sequence coding the carboxy terminal 61 amino acids of rat basic FGF, the putative upstream intron sequence, and a 3′-noncoding region. The cDNAs encoding rat basic FGF predict a molecule consisting of 154 amino acid residues, which is one amino acid shorter than the human and bovine basic FGF. Otherwise, there are only 5 conservative amino acid substitutions between the rat and the human/bovine sequences.Poly A+RNA from brain cortex and hypothalamus show a single 6.0 kb band that hybridizes to the cloned cDNA probe by Northern analyses. The observation that basic FGF mRNA is below the limits of detection in adrenal, spleen, heart, lung, kidney, liver, stomach, small intestine, large intestine, testis, and ovary support the notion that the high levels of the protein found in these tissues is due to storage of the mitogen in the extracellular matrix and not continuous gene expression. The significance of the abundance of mRNA in tissues which are not undergoing either active angiogenesis or cell proliferation (hypothalamus and brain cortex) is unclear but emphasizes the potential neuronotrophic function of basic FGF.