Manipulating cell flocculation-associated protein kinases in Saccharomyces cerevisiae enables improved stress tolerance and efficient cellulosic ethanol production.
Manipulating cell flocculation-associated protein kinases in Saccharomyces cerevisiae enables improved stress tolerance and efficient cellulosic ethanol production.
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DOI：
10.1016/j.biortech.2022.126758
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发表时间：
2022-02
影响因子：
11.4
通讯作者：
Pei-Liang Ye;Xueqing Wang;Bing-Bing Yuan-Bing;Chen-Guang Liu;Xinqing Zhao
中科院分区：
文献类型：
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作者：
Pei-Liang Ye;Xueqing Wang;Bing-Bing Yuan-Bing;Chen-Guang Liu;Xinqing Zhao
细胞自絮凝使酵母菌株具有更好的环境耐受性，有利于生物生产。探索絮凝细胞与非絮凝细胞之间代谢和调控网络的差异，有助于培养具有满意发酵效率的菌株。本研究利用絮凝酵母酿酒酵母espsc01及其在醋酸胁迫下生长的非絮凝突变体，对其转录组、蛋白质组和磷酸化蛋白质组进行了综合分析，结果显示其蛋白激酶发生了显著变化。絮凝上调丝裂原活化蛋白激酶Hog1的过表达导致ROS积累减少，谷胱甘肽过氧化物酶活性增加，从而提高应激条件下乙醇产量。在7个编码蛋白激酶的基因中，akl1在过表达时表现最佳，在玉米芯水解液和模拟玉米秸秆水解液中均具有较高的乙醇产量。这些结果为通过工程关键蛋白激酶inS提高纤维素乙醇产量提供了替代策略。酵母。
Cell self-flocculation endows yeast strains with improved environmental stress tolerance that benefits bioproduction. Exploration of the metabolic and regulatory network differences between the flocculating and non-flocculating cells is conducive to developing strains with satisfactory fermentation efficiency. In this work, integrated analyses of transcriptome, proteome, and phosphoproteome were performed using flocculating yeastSaccharomyces cerevisiaeSPSC01 and its non-flocculating mutant grown under acetic acid stress, and the results revealed prominent changes in protein kinases. Overexpressing the mitogen-activated protein kinase Hog1 upregulated by flocculation led to reduced ROS accumulation and increased glutathione peroxidase activity, leading to improved ethanol production under stress. Among the seven genes encoding protein kinases that were tested,AKL1showed the best performance when overexpressed, achieving higher ethanol productivity in both corncob hydrolysate and simulated corn stover hydrolysate. These results provide alternative strategies for improving cellulosic ethanol production by engineering key protein kinases inS. cerevisiae.