Reduced Vitamin D Receptor on Circulating Endothelial Progenitor Cells: A New Risk Factor of Coronary Artery Diseases.
Reduced Vitamin D Receptor on Circulating Endothelial Progenitor Cells: A New Risk Factor of Coronary Artery Diseases.
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循环内皮祖细胞上维生素 D 受体减少：冠状动脉疾病的新危险因素
DOI：
10.5551/jat.40808
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发表时间：
2018-05-01
影响因子：
4.4
通讯作者：
Liang C
中科院分区：
文献类型：
--
作者：
Ai S;He Z;Ding R;Wu F;Huang Z;Wang J;Huang S;Dai X;Zhang J;Chen J;Liu L;Wu Z;Liang C
目的：内皮祖细胞参与动脉粥样硬化的病理过程。而维生素D及其受体轴可能对EPCs的功能有一定影响。但它们与临床患者的确切关系仍然难以捉摸，这启发了我们探索冠状动脉疾病（CAD）患者循环EPCs中维生素D受体（VDR）表达与血清维生素D水平的潜在关联。方法：选取200例入院后冠心病患者和100例健康对照。检索病史资料并采集新鲜血液样本进行流式细胞术分析。按照标准化方案评价EPCs上VDR的表达。采用Logistic回归分析探讨冠心病的潜在危险因素。结果：冠心病患者的log10vdr - mfi低于对照组，特别是糖尿病患者（p < 0.001）。log10vdr - mfi与糖化血红蛋白呈负相关（R = - 0.472, p < 0.001），而高糖刺激的EPCs的VDR表达较低。多因素logistic回归分析显示，较低的log10vdr - mfi与CAD风险独立相关（OR = 0.055, p = 0.008）。结论：在冠心病患者中，尤其是糖尿病患者中，循环EPCs中VDR表达明显降低。EPCs的VDR表达与HbA1c呈独立负相关，高糖可降低EPCs的VDR表达。循环EPCs中低水平的VDR表达可能是冠心病的潜在危险因素。
Aim: Endothelial progenitor cells (EPCs) are shown to participate in the pathological processes of atherosclerosis. While Vitamin D and its receptor axis might exert some effects on EPCs' function. But their exact relationship with clinical patients is still elusive, which inspired us to explore the potential association of vitamin D receptor (VDR) expression on circulating EPCs and serum vitamin D levels among patients with coronary artery disease (CAD). Methods: Two hundred patients with CAD after their admission to hospital and one hundred healthy controls were enrolled. Medical history data were retrieved and fresh blood samples were collected for flow cytometry analysis. VDR expressions on EPCs were evaluated according to the standardized protocol. Logistic regression analysis was used to investigate the potential risk factor of CAD. Results: CAD patients were found to have lower log10VDR-MFIs than those of control group, especially for patients with diabetes (p < 0.001). Log10VDR-MFIs were inversely correlated with glycated hemoglobin (R = −0.472, p < 0.001), and while EPCs challenged with high glucose had lower VDR expression. Multivariate logistic regression analysis revealed that lower log10VDR-MFIs were independently associated with the risk of CAD (OR = 0.055, p = 0.008). Conclusion: A significant decrease of VDR expression on circulating EPCs was observed among CAD patients, particularly among those also with diabetes. VDR expression on EPCs was independently negatively correlated with HbA1c and high glucose decreased EPCs' VDR expression. Low levels of VDR expression on circulating EPCs might serve as a potential risk factor of CAD.