Glutathione in Synechocystis 6803 A closer look into the physiology of ΔgshB mutant
Glutathione in Synechocystis 6803 A closer look into the physiology of ΔgshB mutant
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DOI：
10.4161/psb.6.1.14145
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发表时间：
2011-01-01
影响因子：
2.9
通讯作者：
Pakrasi, Himadri B.
中科院分区：
文献类型：
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作者：
Cameron, Jeffrey C.;Pakrasi, Himadri B.
谷胱甘肽（GSH）是一种低分子量的巯基化合物，在光合生物中起着许多作用。我们利用Delta gshB（谷胱甘肽合成酶）突变株作为一种工具，以评估GSH在蓝细菌集胞藻PCC 6803（以下简称集胞藻6803），一种模式光合生物的作用。Delta gshB突变体不合成谷胱甘肽，而是将GSH前体γ-谷氨酰半胱氨酸（γ-EC）积累到毫摩尔水平。我们发现，γ-EC是足以允许细胞增殖的最佳条件下，但不是当细胞暴露于促进氧化应激的条件。此外，我们发现，许多影响生长速率和光合活性的因素强烈影响细胞巯基含量。在这里，我们提供了一些额外的见解谷胱甘肽和γ-EC在集胞藻6803的作用，在促进氧化应激的条件。
Glutathione (GSH) is a low molecular weight thiol compound that plays many roles in photosynthetic organisms. We utilized Delta gshB (glutathione synthetase) mutant strain as a tool to evaluate the role of GSH in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis 6803), a model photosynthetic organism. The Delta gshB mutant does not synthesize glutathione, but instead accumulates the GSH precursor, gamma-glutamylcysteine (gamma-EC), to millimolar levels. We found that gamma-EC was sufficient to permit cellular proliferation during optimal conditions, but not when cells were exposed to conditions promoting oxidative stress. Furthermore, we found that many factors affecting growth rate and photosynthetic activities strongly influenced cellular thiol content. Here, we are providing some additional insights into the role of GSH and gamma-EC in Synechocystis 6803 during conditions promoting oxidative stress.