FACTORS INFLUENCING EXIT OF SUBSTANCES FROM CEREBROSPINAL-FLUID INTO DEEP CERVICAL LYMPH OF THE RABBIT
FACTORS INFLUENCING EXIT OF SUBSTANCES FROM CEREBROSPINAL-FLUID INTO DEEP CERVICAL LYMPH OF THE RABBIT
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DOI：
10.1113/jphysiol.1983.sp014731
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发表时间：
1983-01-01
影响因子：
5.5
通讯作者：
WESTROP, RJ
中科院分区：
文献类型：
--
作者：
BRADBURY, MWB;WESTROP, RJ
进行实验以确定放射性碘白蛋白从麻醉兔体内到达颈深部淋巴液（CSF）的主要途径。研究了影响通过该途径排水的其他因素。将[125I]白蛋白单次注射到对照兔的侧脑室后，在6小时内，一侧插管颈静脉干的淋巴中恢复了平均14.8%的脑CSF损失的放射性。在设计用于封闭筛板的程序后2-3周，将高岭土注射到嗅窝或用氰基丙烯酸酯胶密封筛板将[125I]白蛋白的回收率分别降低至3.3和1.9%。这证实了传统观点，即脑脊液和颈深部淋巴之间的主要连接是通过嗅神经周围的蛛网膜下腔的延长，通向鼻粘膜下层的间隙。众所周知，该组织中的致密淋巴丛会流入咽后（颈深部）淋巴结。以10μl/min或30μl/min将人工CSF持续输注到侧脑室中，以便分别使通过系统的流量大约加倍或四倍，将心室内[125I]白蛋白的回收率分别降低至8.1%和6.9%。增加的脑脊液压力迫使相对更多的放射性从颅骨内部进入脊髓的脑脊液空间。保持兔子俯卧，但温度为 20 度。水平方向的倾斜导致淋巴中 17.6% 的 [125I] 白蛋白回收率（头朝上位置和 6.6% 头朝下位置）。低头位时，鼻子和脊髓的放射性量分别显着增加和减少。他们在平视位置上朝相反的方向改变。脑室内注射后，颈深部淋巴中[51Cr]EDTA、[125I]甲曲酰胺和[14C]菊粉的量可以忽略不计。对进入淋巴途径的各种组织中[51Cr]EDTA/[125I]白蛋白比率的估计，以及跨咽后淋巴结的动静脉通量的测量，表明[51Cr]EDTA从脑脊液/淋巴进入鼻子和淋巴结内的血液。脑室内注射后，6小时时颈深部淋巴中[14C]甲基化碳酸酐酶(MW 30,000)的恢复率为4.7%，而[14C]甲基化肌球蛋白(MW 200,000)的恢复率为2.4%。淋巴中[14C]肌球蛋白相对于[125]白蛋白的回收率非常低，这与咽后淋巴结中[14C]肌球蛋白的大量积累有关。结果证实了从筛板穿过鼻粘膜下层到颈深淋巴的通路在兔脑脊液引流中的重要性。尺寸达到并包括菊粉（MW 5,000）的分子通过穿过鼻子和淋巴结中的微血管壁返回血液，而大分子可以通过通往结后淋巴的路径或在途中被细胞吸收。
Experiments were made to determine the main route by which radioiodinated albumin reaches deep cervical lymph from (CSF) in the anesthetized rabbit. Other factors, influencing drainage through this pathway, were investigated. After single injection of [125I]albumin into a lateral ventricle of control rabbits, a mean of 14.8% of the radioactivity lost from brain CSF was recovered during 6 h in the lymph of the cannulated jugular trunk of 1 side. Injection of kaolin into the olfactory fossa or sealing of the cribriform plate with cyanoacrylate glue reduced the recovery of [125I]albumin to 3.3 and 1.9%, respectively, at 2-3 wk after the procedure designed to block the cribriform plate. This confirms the traditional view that the major connections between CSF and deep cervical lymph is via prolongations of subarachnoid space around the olfactory nerves, leading into the interstitial spaces of the nasal submucosa. The dense lymphatic plexus in this tissue is known to drain into the retropharyngeal (deep cervical) lymph nodes. Constant infusion of artificial CSF into a lateral ventricle at 10 .mu.l/min or 30 .mu.l/min, in order to approximately double or quadruple flow through the system respectively, decreased the recovery of intraventricular [125I]albumin to 8.1 and 6.9%, respectively. The increased CSF pressures induced forced relatively more radioactivity from inside the skull into the CSF spaces of the spinal cord. Maintaining the rabbit prone but at 20.degree. from the horizontal caused recoveries of [125I]albumin in lymph of 17.6% (head-up position and 6.6% head-down). The amounts of radioactivity in nose and spinal cord markedly increased and decreased respectively in the head-down position. They changed in the opposite directions in the head-up position. The amounts of [51Cr]EDTA, [125I]metrizamide and [14C]inulin in deep cervical lymph were negligible after intraventricular injection. Estimations of the ratio of [51Cr]EDTA/[125I]albumin in various tissues on the pathway into lymph, together with measurements of arterio-venous fluxes across the retropharyngeal nodes, indicate that [51Cr]EDTA passed from CSF/lymph into blood within both the nose and the lymph nodes. After intraventricular injection, the recovery of [14C]methylated carbonic anhydrase (MW 30,000) in deep cervical lymph was 4.7% at 6 h, whereas that of [14C]methylated myosin (MW 200,000) was 2.4%. The very low recovery of [14C]myosin relative to [125]albumin in lymph was associated with a large accumulation of the [14C]myosin in the retropharyngeal lymph nodes. The results confirm the importance of a pathway from cribriform plate, through nasal submucosa to deep cervical lymph in drainage of CSF in the rabbit. Molecules up to and including inulin in size (MW 5,000) return to blood by crossing microvessel walls in the nose and lymph nodes, whereas macromolecules may pass through the pathway to post-nodal lymph or be taken up by cells en route.