RESISTANCE OF THE MOUSE'S INTESTINAL TRACT TO EXPERIMENTAL SALMONELLA INFECTION. I. FACTORS WHICH INTERFERE WITH THE INITIATION OF INFECTION BY ORAL INOCULATION.
RESISTANCE OF THE MOUSE'S INTESTINAL TRACT TO EXPERIMENTAL SALMONELLA INFECTION. I. FACTORS WHICH INTERFERE WITH THE INITIATION OF INFECTION BY ORAL INOCULATION.
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小鼠肠道对实验沙门氏菌感染的抗性。 I.干扰口服接种感染的因素。
DOI：
10.1084/jem.120.5.805
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发表时间：
1964-11-01
影响因子：
15.3
通讯作者：
MARTIN, W R
中科院分区：
文献类型：
--
作者：
BOHNHOFF, M;MILLER, C P;MARTIN, W R
从正常小鼠大肠中新鲜取出的粪便物质的缓冲悬浮液在体外抑制沙门氏菌的增殖。最有效的是从盲肠和横结肠获得的材料。通过加热或过滤灭菌不会损害抑制活性。从这些材料中分离出乙酸和丁酸，其浓度在体外抑制沙门氏菌。结肠内容物和两种脂肪酸的混合物的悬浮液的抑制活性的程度由pH调节，并有利于厌氧。通过直接测量原位测定，在大多数正常小鼠结肠内容物的pH值或略低于pH值时发生有效抑制。乙酸和丁酸是从厌氧培养的几个菌株的拟杆菌以前被证明是最多的居民之一的大肠正常小鼠。
Multiplication of Salmonella enteritidis was inhibited in vitro by buffered suspensions of fecal material freshly removed from the large intestine of normal mice. Most effective was material obtained from cecum and transverse colon. Inhibitory activity was not impaired by sterilization by heat or filtration. From such materials were isolated acetic and butyric acids in concentrations which inhibited Salmonella in vitro. The degree of inhibitory activity of suspensions of colon content and of mixtures of the two fatty acids was conditioned by pH and favored by anaerobiosis. Effective inhibition occurred at or slightly below the pH of colon content of most normal mice as determined in situ by direct measurement. Acetic and butyric acids were isolated from anaerobic cultures of several strains of Bacteroides previously demonstrated to be one of the most numerous inhabitants of the large intestine of the normal mouse.