Novel single-nucleotide variations associated with vancomycin resistance in vancomycin-intermediate Staphylococcus aureus.
Novel single-nucleotide variations associated with vancomycin resistance in vancomycin-intermediate Staphylococcus aureus.
复制标题
新型的单核苷酸变异与万古霉素中间葡萄球菌葡萄球菌中的万古霉素抗性有关。
DOI：
10.2147/idr.s148335
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发表时间：
2018
影响因子：
3.9
通讯作者：
Lu JJ
中科院分区：
文献类型：
--
作者：
Lin LC;Chang SC;Ge MC;Liu TP;Lu JJ
万古霉素长期使用可能导致耐甲氧西林金黄色葡萄球菌变成万古霉素中间体S。金黄色葡萄球菌（VISA）和异质VISA（hVISA）。VISA和hVISA的万古霉素耐药机制尚不清楚。本研究分析了30株万古霉素敏感性链球菌的核苷酸序列变异。41株hVISA和16株VISA分离株在与细胞壁合成或双组分系统相关的12个基因（fmtC、graR、graS、htrA、mecA、pbp 2、pbp 4、srtA、tcaA、upps、vicK和vraR）上发现了29个单核苷酸变异。这29个单核苷酸变异中有6个是新的，并导致以下氨基酸变化：FmtC中的Q692 E; HtrA中的T278 I，P306 L和I311 T;以及Upps中的I63 V和K101 E。由于大多数（76.7%-86.7%）VSSA分离株中存在HtrA中的P306 L和I311 T以及Ups中的I63 V，因此这三种氨基酸变异可能与万古霉素耐药性无关。其他三种氨基酸变异（HtrA中的T278 I、Ups中的K101 E和FmtC中的Q692 E）在大多数（87.5%-93.8%）hVISA和VISA分离株中存在，但仅在少数（22.9%-25.7%）VSSA分离株中存在，表明它们与万古霉素耐药相关。
Prolonged vancomycin usage may cause methicillin-resistant Staphylococcus aureus to become vancomycin-intermediate S. aureus (VISA) and heterogeneous VISA (hVISA). Mechanisms of vancomycin resistance of VISA and hVISA are still unclear. In this study, analyses of nucleotide sequence variations in 30 vancomycin-sensitive S. aureus (VSSA), 41 hVISA and 16 VISA isolates revealed 29 single-nucleotide variations in 12 genes (fmtC, graR, graS, htrA, mecA, pbp2, pbp4, srtA, tcaA, upps, vicK and vraR) that are related to cell wall synthesis or the two-component system. Six of these 29 single-nucleotide variations were novel and resulted in the following amino acid changes: Q692E in FmtC; T278I, P306L and I311T in HtrA; and I63V and K101E in Upps. Since P306L and I311T in HtrA and I63V in Upps were present in the majority (76.7%–86.7%) of VSSA isolates, these three amino acid variations may not be associated with vancomycin resistance. The other three amino acid variations (T278I in HtrA, K101E in Upps and Q692E in FmtC) were present in the majority (87.5%–93.8%) of hVISA and VISA isolates, but only in a small number (22.9%–25.7%) of VSSA isolates, suggesting that they are associated with vancomycin resistance.