Polymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) around living neural cells
Polymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) around living neural cells
复制标题
DOI：
10.1016/j.biomaterials.2006.11.026
复制
发表时间：
2007-03-01
期刊：
影响因子：
14
通讯作者：
Martin, David C.
中科院分区：
文献类型：
--
作者：
Richardson-Burns, Sarah M.;Hendricks, Jeffrey L.;Martin, David C.
在本文中，我们描述了神经细胞和导电聚合物聚（3，4-乙撑二氧噻吩）（PEDOT）之间的相互作用，以开发用于与电活性组织（如神经系统，心脏和骨骼肌）直接，功能性接触的导电生物材料。我们介绍了一种在活细胞周围聚合PEDOT的方法，并描述了一种用于微电极的神经细胞模板导电聚合物涂层和一种混合导电聚合物-活神经细胞电极。我们发现，神经细胞可以暴露于工作浓度（0.01 μ M）的EDOT单体长达72它，同时保持80%的细胞活力。使用0.5-1 μ A/mm（2）恒电流，PE-DOT可以电化学沉积在电极上培养的神经元周围。PEDOT在电极上聚合并包围细胞，覆盖细胞突起。在细胞很好地粘附到基底的区域中聚合受到阻碍。可以从PEDOT基质中去除细胞以产生具有吸引细胞的细胞形状特征的神经细胞模板仿生导电基底。嵌入导电聚合物基质内的活细胞在聚合后保持活力至少120小时。死亡细胞主要经历凋亡性细胞死亡。与裸电极相比，电极上的PEDOT、PEDOT +活神经元和神经元模板化PEDOT涂层显著增强了电性能，如在0.01-1 kHz下电阻抗降低1-1.5个数量级和电荷转移容量显著增加所示。PEDOT涂层显示阻抗的相位角从裸电极的大约80度降低到在> 0.1kHz的频率下的5-35度。等效电路模型表明，PEDOT涂层电极的最佳描述R（C（RT））电路。我们发现，一个RC并联电路必须添加到模型的PEDOT+活神经元和神经元模板PEDOT涂层。(c)2006爱思唯尔有限公司版权所有。
In this paper, we describe interactions between neural cells and the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) toward development of electrically conductive biomaterials intended for direct, functional contact with electrically active tissues such as the nervous system, heart, and skeletal muscle. We introduce a process for polymerizing PEDOT around livings cells and describe a neural cell-templated conducting polymer coating for microelectrodes and a hybrid conducting polymer-live neural cell electrode. We found that neural cells could be exposed to working concentrations (0.01 m) of the EDOT monomer for as long as 72 It while maintaining 80% cell viability. PE-DOT could be electrochemically deposited around neurons cultured on electrodes using 0.5-1 mu A/mm(2) galvanostatic Current. PEDOT polymerized on the electrode and surrounded the cells, covering cell processes. The polymerization was impeded in regions where cells were well adhered to the substrate. The cells could be removed from the PEDOT matrix to generate a neural cell-templated biomimetic conductive substrate with cell-shaped features that were cell attracting. Live cells embedded within the conductive polymer matrix remained viable for at least 120 h following polymerization. Dying cells primarily underwent apoptotic cell death. PEDOT, PEDOT + live neurons, and neuron-templated PEDOT coatings on electrodes significantly enhanced the electrical properties as compared to the bare electrode as indicated by decreased electrical impedance of 1-1.5 orders of magnitude at 0.01-1 kHz and significantly increased charge transfer capacity. PEDOT coatings showed a decrease of the phase angle of the impedance from roughly 80 degrees for the bare electrode to 5-35 degrees at frequencies > 0.1 kHz. Equivalent circuit modeling indicated that PEDOT-coated electrodes were best described by R(C(RT)) circuit. We found that an RC parallel circuit must be added to the model for PEDOT+live neuron and neuron-templated PEDOT coatings. (c) 2006 Elsevier Ltd. All rights reserved.