Peripheral kisspeptin reverses short photoperiod-induced gonadal regression in Syrian hamsters by promoting GNRH release
Peripheral kisspeptin reverses short photoperiod-induced gonadal regression in Syrian hamsters by promoting GNRH release
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DOI：
10.1530/rep-10-0313
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发表时间：
2011-09-01
期刊：
影响因子：
3.8
通讯作者：
Simonneaux, V.
中科院分区：
文献类型：
--
作者：
Ansel, L.;Bentsen, A. H.;Simonneaux, V.
在季节性繁殖中，繁殖通过松果体激素褪黑激素根据日长进行同步。在短冬日（短日，SD）中，叙利亚仓鼠表现出性腺完全萎缩，同时 Kisspeptins (Kp) 的表达显着减少，Kisspeptins 是 GNRH 神经元的下丘脑强刺激剂家族。据报道，中枢和外周急性注射 Kp 均可激活哺乳动物的促性腺轴。本研究的目的是确定 Kp54 的外周给药是否以及如何能够恢复光抑制仓鼠的性腺功能。每天两次腹腔注射重新激活保存在 SD 中的仓鼠的睾丸活动。注射 Kp54，但不是通过微型泵长期皮下输送相同的肽。急性腹腔注射注射 Kp54 诱导大量 GNRH 神经元和垂体促性腺激素表达 FOS（c-Fos），同时循环睾酮强烈增加。预先给予 GNRH 受体拮抗剂 acyline 可以抑制 Kp 对垂体细胞的激活。总而言之，我们的结果表明，外周 Kp54 通过刺激 GNRH 释放来激活促性腺轴，并表明长期全身性 Kp 给药的适当方案可以重新产生光抑制的生殖轴。复制（2011）142 417-425
In seasonal breeders, reproduction is synchronised by day length via the pineal hormone melatonin. In short winter days (short day, SD), the Syrian hamster displays a complete gonadal atrophy together with a marked reduction in expression of kisspeptins (Kp), a family of potent hypothalamic stimulators of GNRH neurons. Both central and peripheral acute injections of Kp have been reported to activate the gonadotropic axis in mammals. The aim of this study was to determine if and how peripheral administration of Kp54 could restore gonadal function in photo-inhibited hamsters. Testicular activity of hamsters kept in SD was reactivated by two daily i.p. injections of Kp54 but not by chronic subcutaneous delivery of the same peptide via mini-pumps. Acute i.p. injection of Kp54-induced FOS (c-Fos) expression in a large number of GNRH neurons and pituitary gonadotrophs together with a strong increase in circulating testosterone. The activation of pituitary cells by Kp was inhibited by preadministration of the GNRH receptor antagonist acyline. Altogether, our results demonstrate that peripheral Kp54 activates the gonadotropic axis by stimulating GNRH release and indicate that an appropriate protocol of long-term systemic Kp administration can recrudesce a photo-inhibited reproductive axis. Reproduction (2011) 142 417-425