Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients
Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients
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DOI：
10.1016/j.tube.2016.07.004
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发表时间：
2016-09-01
期刊：
影响因子：
3.2
通讯作者：
Walter, Nicholas D.
中科院分区：
文献类型：
--
作者：
Garcia, Benjamin J.;Loxton, Andre G.;Walter, Nicholas D.
人痰中病原体靶向转录谱可能阐明结核分枝杆菌的生理状态。在感染和治疗过程中。然而，M.痰中的结核转录重现肺中的转录是不确定的。我们比较了M。在11例HIV阴性南非肺结核患者的人痰和支气管肺泡灌洗液（BAL）样本中检测到结核转录。我们还将这些临床样品与体外对数期需氧生长和缺氧非复制持久性（NRP-2）进行了比较。在2179米。采用多重RT-PCR检测痰液和BAL中的结核病转录物，194例（8.9%）具有p值
Pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of Mycobacterium tuberculosis (M. tuberculosis) during infection and treatment. However, whether M. tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain. We therefore compared M. tuberculosis transcription in human sputum and bronchoalveolar lavage (BAL) samples from 11 HIV-negative South African patients with pulmonary tuberculosis. We additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic non-replicating persistence (NRP-2). Of 2179 M. tuberculosis transcripts assayed in sputum and BAL via multiplex RT-PCR, 194 (8.9%) had a p-value