Preferential incorporation of shorter and less unsaturated acyl phospholipids into high density lipoprotein-like particles in the ABCA1-and ABCA7-mediated biogenesis with apoA-I
Preferential incorporation of shorter and less unsaturated acyl phospholipids into high density lipoprotein-like particles in the ABCA1-and ABCA7-mediated biogenesis with apoA-I
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DOI：
10.1016/j.chemphyslip.2015.01.005
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发表时间：
2015-04-01
影响因子：
3.4
通讯作者：
Yokoyama, Shinji
中科院分区：
文献类型：
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作者：
Hotta, Noriko;Abe-Dohmae, Sumiko;Yokoyama, Shinji
在用人载脂蛋白A-I（apoA-I）形成的新生高密度脂蛋白（HDL）样颗粒中，对磷脂酰胆碱（PC）和鞘磷脂（SPM）的分子种类进行脂质组学的全局分析，其中人ATP结合盒转运体（ABC）A1或ABCA 7被转染并过表达。SPM/PC比率在ABCA 1介导的HDL中高于ABCA 7介导的HDL，这可能与它们的胆固醇含量有关，而在任一情况下其均小于细胞膜中的比率。HDL中各磷脂中烃链部分的分子种类组成在很大程度上反映了脂蛋白来源于细胞，ABCA 1和ABCA 7之间无显著差异。然而，进一步的分析显示，在通过ABCA 1和ABCA 7相对并入HDL中时，PC和SPM明显偏好具有较短烃链长度的分子。同样，它有利于PC的不饱和烃链，而SPM的这种偏好不明显。结果是一致的，认为HDL颗粒与细胞外apoA-I的组装主要是与细胞磷脂分子被调节，部分由其物理化学性质。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Molecular species of phosphatidylcholine (PC) and sphingomyelin (SPM) were globally analyzed for lipidomics in the nascent high-density lipoprotein (HDL)-like particles generated with human apolipoprotein A-I (apoA-I) form HEK293 cells where either human ATP binding cassette transporter (ABC) A1 or ABCA7 was transfected and overexpressed. SPM/PC ratio was higher in the ABCA1-mediated HDL than ABCA7-mediated HDL likely being related to their cholesterol content, while it was less than the ratio in the cell membrane in either case. Molecular species composition of hydrocarbon chain moiety in each phospholipid in the HDL largely reflected that in the cells the lipoprotein originated in, without remarkable difference between ABCA1 and ABCA7. Further analysis, however, revealed apparent preference for the molecules with shorter hydrocarbon chain length for both PC and SPM in their relative incorporation into HDL by ABCA1 and ABCA7. Likewise, it was in favor for less-unsaturated hydrocarbon chains of PC while this preference was not apparent for SPM. The results are consistent with the view that assembly of HDL particles with extracellular apoA-I is primarily with the cellular phospholipid molecules being regulated in part by their physicochemical nature. (C) 2015 Elsevier Ireland Ltd. All rights reserved.