Biological decolorization of dye solution containing malachite green by Pandoraea pulmonicola YC32 using a batch and continuous system
Biological decolorization of dye solution containing malachite green by Pandoraea pulmonicola YC32 using a batch and continuous system
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DOI：
10.1016/j.jhazmat.2009.08.009
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发表时间：
2009-12-30
影响因子：
13.6
通讯作者：
Chung, Ying-Chien
中科院分区：
文献类型：
--
作者：
Chen, Chih-Yu;Kuo, Jong-Tar;Chung, Ying-Chien
在我们的研究中，我们已经分离出一种相对较新鉴定的细菌物种，Pandoraea pulmonicola YC 32，并首次评估其治疗孔雀石绿色（MG）的能力。在间歇系统中考察了各种因素对脱色效果的影响。在pH为7-10范围内，脱色率最高，当MG初始浓度为100 mg/l时，脱色率随MG初始浓度的增加而增加。脱色率与MG浓度的关系符合Lineweaver-Burk方程。表观动力学参数R-MG、R-max和K-m分别为6.23 mg-MG/g-cell/h和153.4mg/l。分别肺曲霉YC 32降解MG的起始步骤是还原或N-脱甲基反应。在固定化肺曲霉YC 32连续柱系统中，MG浓度为50 mg/l时，脱色率为85.2%。这是第一个报告的应用程序的连续柱系统，以脱色MG使用的微生物。(C)2009爱思唯尔有限公司版权所有。
In our study, we have isolated a relatively newly identified bacteria species, Pandoraea pulmonicola YC32, and first assessed its capability to treat malachite green (MG). The effects of various factors on decolorization efficiency were investigated in a batch system. The decolorization efficiency was found to be optimal within a pH of 7-10 and it increased, with increasing initial MG concentration up to 100mg/l. The relationship between the decolorization rate and MG concentration agreed with Lineweaver-Burk equation. The apparent kinetic parameters, R-MG,R-max and K-m, were 6.23 mg-MG/g-cell/h and 153.4mg/l. respectively. The initial step in the biodegradation pathway of MG by A pulmonicola YC32 was a reduction or N-demethylation reaction. We achieved a decolorization efficiency of 85.2% with 50 mg/l MG in the immobilized A pulmonicola YC32 continuous column system. This is the first report on the application of a continuous column system to decolorize MG using a microorganism. (C) 2009 Elsevier B.V. All rights reserved.