High abundance of Lachnospiraceae in the human gut microbiome is related to high immunoscores in advanced colorectal cancer
High abundance of Lachnospiraceae in the human gut microbiome is related to high immunoscores in advanced colorectal cancer
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DOI：
10.1007/s00262-022-03256-8
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发表时间：
2022-07
期刊：
影响因子：
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通讯作者：
Zhang Hexun;Toru Miyake;Takeru Maekawa;Haruki Mori;Daiki Yasukawa;Masashi Ohno;A. Nishida;A. Andoh;M. Tani
中科院分区：
文献类型：
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作者：
Zhang Hexun;Toru Miyake;Takeru Maekawa;Haruki Mori;Daiki Yasukawa;Masashi Ohno;A. Nishida;A. Andoh;M. Tani
结直肠癌的肿瘤微环境(TME)包括肠道微生物组、免疫细胞、血管生成因子和成纤维细胞，在肿瘤进展中起重要作用。免疫核心(IS)是基于免疫细胞对肿瘤的侵袭，免疫细胞是已知的CRC预后生物标记物。然而，人类结直肠癌中IS、微生物组和其他TME因素之间的相互关系仍不清楚。患者和方法在日本滋贺医科大学医院对94名结直肠癌患者进行了队列检查。采用免疫组织化学方法检测CD3、CD8、CD31和α-平滑肌肌动蛋白(α-SMA)在原发肿瘤中的表达水平。根据CD3和CD8染色结果计算IS。结果α-SMA与肿瘤浸润性淋巴细胞的表达水平呈负相关(P= 0.006)。结论癌相关成纤维细胞密度降低和肠道菌群富集是原发肿瘤淋巴细胞浸润的特点，这可能是影响大肠癌进展的重要因素。
IntroductionThe tumor microenvironment (TME) in colorectal cancer (CRC) includes the gut microbiome, immune cells, angiogenic factors, and fibroblasts and plays a major role in cancer progression. The Immunoscore (IS) is based on tumor infiltration by immune cells that are known prognostic biomarkers for CRC. However, the interrelation between the IS, microbiome, and other TME factors in human CRC remains unclear.Patients and methodsA cohort of 94 patients with CRC was examined at the Shiga University of Medical Science Hospital in Japan. The expression levels of CD3, CD8, CD31, and alpha-smooth muscle actin (α-SMA) in the primary tumor were evaluated by immunohistochemistry. The IS was calculated based on the results of the CD3 and CD8 staining assays. Microbiomes in patients with CRC were examined by amplicon sequencing.ResultsThe expression levels of α-SMA and tumor-infiltrating lymphocytes in patients with CRC were negatively correlated (P= 0.006). A high IS was associated with high abundance of Lachnospiraceae in the microbiomes of patients with CRC.ConclusionLymphocyte infiltration into the primary tumor was marked by reduced density of cancer-associated fibroblasts and enrichment of the Lachnospiraceae family in the gut microbiome, which may influence CRC progression.