Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression
Tissue digestion with dispase substantially reduces lymphocyte and macrophage cell-surface antigen expression
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DOI：
10.1016/0022-1759(96)00067-1
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发表时间：
1996-07-17
影响因子：
2.2
通讯作者：
Sedgwick, JD
中科院分区：
文献类型：
--
作者：
Ford, AL;Foulcher, E;Sedgwick, JD
使用Dispase消化中枢神经系统（CNS）组织，以分离固定的组织巨噬细胞群（CNS小胶质细胞）和存在的其他白细胞。在酶处理前，通过将已知表型的CD 45（B）同种异型标记的后活化CD 4（+）T细胞添加到源自CD 45（a）表达大鼠品系的CNS组织制备物中，进一步研究了某些白细胞表面标志物表达的明显降低。在完成分离程序后，对这些T细胞的一系列表面分子表达的评估显示，CD4和CD25的表达几乎完全丧失，并且许多其他表面抗原的表达减少。
Dispase was used to digest central nervous system (CNS) tissue for isolation of the fixed tissue macrophage population (CNS microglia) and other leucocytes present. An apparent reduction in expression of some leucocyte cell surface markers was investigated further by addition of CD45(b) allotype-marked post-activated CD4(+) T cells of known phenotype to CNS tissue preparations derived from a CD45(a)-expressing rat strain, before enzymatic treatment. Assessment of these T cells for expression of a range of surface molecules after completion of the isolation procedure revealed almost complete loss of expression of CD4 and CD25 and reduced expression of a number of other surface antigens.