Manipulation of plasma membrane fatty acid composition of fetal rat brain cells grown in a serum-free defined medium.
Manipulation of plasma membrane fatty acid composition of fetal rat brain cells grown in a serum-free defined medium.
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操纵在无血清成分确定的培养基中生长的胎鼠脑细胞的质膜脂肪酸组成。
DOI：
10.1111/j.1471-4159.1990.tb04936.x
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发表时间：
1990
影响因子：
4.7
通讯作者：
Ahmed,Z
中科院分区：
文献类型：
--
作者：
Park,CC;Hennessey,T;Ahmed,Z
通过在培养基中添加必需脂肪酸，对质膜酰基连接的磷脂脂肪酸组成进行了修饰。用Percoll梯度离心法从无血清培养的分离的胎鼠脑细胞中纯化出质膜部分。研究了修饰的浓度依赖性和时间过程。添加必需多不饱和脂肪酸、亚麻酸(18：3ω3)或亚油酸(18：2ω6)时，ω3或ω6类的伸长产物和去饱和产物分别被掺入，即掺入具有类特异性。在每一类中，最不饱和和最细长的成员，即末端成员，优先结合，直到它们在6-7天内达到最大浓度。当添加较高浓度的脂肪酸时，通过增加中间成员的浓度而在质膜上产生额外的类特异性掺入。与此同时，单不饱和脂肪酸的浓度下降，饱和脂肪酸的浓度保持不变。脂肪酸组成的改变是可逆的，时间进程类似于掺入。细胞质膜总磷脂和甾醇含量不随脂肪酸组成的变化而变化，但随培养时间的延长而变化。这种制备方法将有助于研究多不饱和脂肪酸在脑细胞功能中的作用，包括神经元的兴奋性。
Modifications of plasma membrane acyl‐linked phospholipid fatty acid composition were produced by supplementing the culture medium with essential fatty acids. The plasma membrane fraction was purified by Percoll gradient centrifugation from dissociated fetal rat brain cells grown in a serum‐free culture medium. Both the concentration dependence and the time course of the modifications were examined. Supplementation of the medium with essential polyunsaturated fatty acid, linolenic acid (18:3ω3) or lin‐oleic acid (18:2ω6), produced incorporation of the elongated and desaturated products of ω3 or ω6 class, respectively, i.e., the incorporation was class specific. Within each class, the most unsaturated and elongated members, i.e., terminal members, were preferentially incorporated until they reached a maximum concentration within 6–7 days. At higher concentrations of supplemented fatty acids, additional class specific incorporation in plasma membrane was produced by an increase in the concentration of intermediate members. At the same time, the concentration of monounsaturated fatty acids declined and that of saturated fatty acids remained unchanged. The modifications in fatty acid composition were reversible, with the time course similar to that of incorporation. The total plasma membrane phospholipid and sterol contents did not change with alterations of fatty acid composition, but did change with time in culture. This preparation should prove useful for investigating the role of polyunsaturated fatty acids in brain cell functions, including neuronal excitability.