Effects of CDC42 on the proliferation and invasion of gastric cancer cells
Effects of CDC42 on the proliferation and invasion of gastric cancer cells
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DOI：
10.3892/mmr.2015.4523
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发表时间：
2016-01-01
影响因子：
3.4
通讯作者：
Tang, Xiao-Jun
中科院分区：
文献类型：
--
作者：
Du, Dong-Shu;Yang, Xiao-Zhong;Tang, Xiao-Jun
细胞分裂周期42（CDC 42）是Rho GT3家族的一员，参与调节多种肿瘤细胞的转移，但其在胃癌中的作用尚不清楚。本研究旨在探讨CDC 42对胃癌细胞增殖、迁移和侵袭的影响。进一步探讨了CDC 42对胃癌细胞生长的影响及其分子机制。通过RNA干扰降低了AGS和SGC 7901人胃癌细胞系中CDC 42的表达水平。下调CDC 42基因可显著抑制AGS和SGC 7901细胞的增殖，其机制可能与细胞周期阻滞于G1/S期，细胞周期蛋白A、D1、E和增殖细胞核抗原表达下调有关。此外，CDC 42基因的敲低可明显抑制胃癌细胞的迁移和侵袭，并抑制基质金属蛋白酶9的表达。这些结果表明，CDC 42是参与调节GC增殖、迁移和侵袭的关键调节因子，其可能被认为是GC的潜在治疗靶点。
Cell division cycle 42 (CDC42), which is a member of the Rho GTPase family, has been reported to regulate the metastasis of various human cancer cells; however, the role of CDC42 in gastric cancer (GC) remains unclear. The present study aimed to investigate the effects of CDC42 on the proliferation, migration and invasion of GC. Furthermore, the molecular mechanisms underlying the effects of CDC42 on GC were explored. The expression levels of CDC42 in the AGS and SGC7901 human GC cell lines were reduced by RNA interference. Knockdown of CDC42 significantly inhibited the proliferation of AGS and SGC7901 cells, and it was suggested that this inhibitory process may be due to cell cycle arrest at G1/S phase and downregulation of cyclin A, cyclin D1, cyclin E and proliferating cell nuclear antigen. Furthermore, knockdown of CDC42 markedly inhibited the migration and invasion of GC cells, and suppressed the expression of matrix metalloproteinase 9. These results indicated that CDC42 is a key regulator involved in regulating the proliferation, migration and invasion of GC, and it may be considered a potential therapeutic target in GC.