What is the nature of the RGC-5 cell line?
What is the nature of the RGC-5 cell line?
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DOI：
10.1007/978-1-4614-3209-8_19
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发表时间：
2014
影响因子：
--
通讯作者：
C. Sippl;E. Tamm
中科院分区：
文献类型：
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作者：
C. Sippl;E. Tamm
RGC-5永生化细胞系已被广泛用于视网膜神经节细胞(RGCs)的神经生物学研究。这些细胞最初是作为大鼠RGC的来源引入的，显示出各种神经元标记物的表达，特别是RGC特征蛋白Brn3和Thy1。最近的数据引起了人们对细胞来源和性质的担忧。RGC-5细胞被鉴定为小鼠来源，其RGC特性的表达受到一些实验室的质疑。本文总结了有关RGC-5特性的现有数据，讨论了区分RGC-5的常见方案，旨在为研究人员提供一些关于RGC-5的优点和局限性的研究指南。
The immortalized RGC-5 cell line has been widely used as a cell culture model to study the neurobiology of retinal ganglion cells (RGCs). The cells were originally introduced as derived from rat RGC showing expression of various neuronal markers, in particular the RGC-characteristic proteins Brn3 and Thy1. Recent data gave rise to concerns regarding the origin and nature of the cells. RGC-5 cells were identified to be of mouse origin and their expression of RGC characteristics was questioned by some laboratories. This article summarizes the available data on the properties of RGC-5, discusses common protocols for their differentiation and is aimed at providing researchers some guidelines on the benefits and limitations of RGC-5 for research.