Trp repressor protein controls its own structural gene.
Trp repressor protein controls its own structural gene.
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Trp阻遏蛋白控制其自身的结构基因。
DOI：
10.1016/0022-2836(81)90361-2
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发表时间：
1981
影响因子：
5.6
通讯作者：
Somerville,R
中科院分区：
文献类型：
--
作者：
Bogosian,G;Bertrand,K;Somerville,R
为研究大肠杆菌trpR基因的表达调控，采用体外切割和连接技术，构建了由trpR基因启动子-操纵子区和一个无启动子但有功能的lacZ基因组成的融合转录单位。在这种类型的融合体中，trpR+单倍体菌株和缺失trpR基因的菌株的β-半乳糖苷酶水平基本相同。当含有trpR-lacZ融合蛋白的菌株用能导致Trp阻遏物过量产生的质粒转化时，β-半乳糖苷酶的产量显著降低。这一结果证实了早期的建议（Singletonet al.，1980），trpR基因是自发调节的。
To study the regulation of expression of thetrpRgene ofEscherichia coli, fused transcriptional units consisting of the promoter-operator region oftrpRand a functional but promoterlesslacZgene were constructed by techniques of cleavage and ligationin vitro. In fusions of this type, the β-galactosidase levels oftrpR+haploid strains and strains deleted for thetrpRgene were essentially identical. Significant reduction of β-galactosidase production resulted when strains harboringtrpR-lacZfusions were transformed with plasmids that cause overproduction of Trp repressor. This result confirms earlier suggestions (Singletonet al., 1980) that thetrpRgene is regulated autogenously.