Sterol-deficient domains correlate with intramembrane particle arrays in the plasma membrane of Chlamydomonas reinhardii.
Sterol-deficient domains correlate with intramembrane particle arrays in the plasma membrane of Chlamydomonas reinhardii.
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甾醇缺陷域与莱茵衣藻质膜中的膜内颗粒阵列相关。
DOI：
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发表时间：
1981
影响因子：
6.6
通讯作者：
M. Melkonian
中科院分区：
文献类型：
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作者：
H. Robenek;M. Melkonian
采用冻裂技术，以多烯抗生素filipin和皂苷番茄素为细胞化学标记物，研究了绿鞭毛衣藻（Chlamydomonas reinhardii）质膜中3-羟基甾醇的平面分布。菲律宾甾醇复合物主要在质膜e面有25 ~ 30 nm的突起，p面有相应的内陷。一般来说，菲律宾甾醇复合物和番茄甾醇复合物是随机分布的，但在膜内颗粒阵列（imp -阵列，即鞭毛项链，鞭毛手镯包括支柱阵列，眼点膜）的位置几乎没有。结果表明，衣藻的imp -阵列缺乏3- β -羟基甾醇，因此可能被视为特殊的脂质阵列。imp阵列可能需要改变脂质环境才能正常发挥作用。讨论了衣藻中imp阵列的一些功能方面。
The planar distribution of 3-beta-hydroxysterols in the plasmalemma of the green flagellate Chlamydomonas reinhardii has been studied with the freeze-fracture technique using the polyene antibiotic filipin and the saponin tomatin as cytochemical markers. Filipin-sterol complexes were predominantly observed as 25 to 30 nm protuberances on the E-face of the plasmalemma with corresponding invaginations on the P-face. Generally filipin-sterol and tomatin-sterol complexes were randomly distributed, but were virtually absent from sites of intramembrane particle arrays (IMP-arrays, i.e. the flagellar necklace, the flagellar bracelet including strut arrays, the eyespot membrane). The results suggest that IMP-arrays of Chlamydomonas are deficient in 3-beta-hydroxysterols and may therefore be regarded as special lipid arrays. IMP-arrays might require an altered lipid environment for proper function. Some functional aspects of IMP-arrays in Chlamydomonas are discussed.