Esterase activity as a novel parameter of spore germination in Bacillus anthracis
Esterase activity as a novel parameter of spore germination in Bacillus anthracis
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DOI：
10.1016/j.bbrc.2004.05.062
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发表时间：
2004-07-02
影响因子：
3.1
通讯作者：
Rotman, B
中科院分区：
文献类型：
--
作者：
Ferencko, L;Cote, MA;Rotman, B
炭疽芽孢杆菌孢子在暴露于氨基酸和嘌呤核苷组合等常规发芽剂后约4分钟产生酯酶活性。氨基酸和核苷都不能单独诱导萌发和酯酶活性。酯酶活性的表达对氯霉素具有抗性，并与孢子折光性的丧失有关，而折光性是早期发芽的传统参数。基于这些观察，我们假设酯酶活性可以作为量化孢子萌发早期事件的新参数。为了验证这一假设，我们测量了不同发芽条件下酯酶活性的表达。使用二乙酰荧光素作为酯酶的荧光底物，我们证明了无论何时孢子被已知的生发物触发，酯酶活性都会被诱导。此外，l -丙氨酸介导的发芽抑制剂d -丙氨酸显著抑制酯酶活性的表达。在分子机制方面，酯酶的表达可能代表了孢子萌发时蛋白酶的激活。(C) 2004爱思唯尔公司版权所有。
Spores of Bacillus anthracis were shown to produce esterase activity about 4 min after exposure to conventional germinants such as combinations of amino acids and purine ribosides. Neither amino acids nor ribosides alone induce germination and esterase activity. Expression of esterase activity was chloramphenicol resistant, and correlated with loss of spore refractivity, a traditional parameter of early germination. Based on these observations, we hypothesized that esterase activity could be used as a novel parameter for quantifying early events during spore germination. To test this hypothesis, we measured expression of esterase activity under a variety of germinating conditions. Using diacetyl fluorescein as fluorogenic substrate of esterases, we demonstrated that esterase activity was invariably induced whenever spores were triggered by known germinants. Moreover, D-alanine, an inhibitor of L-alanine-mediated germination, was found to significantly inhibit expression of esterase activity. In terms of molecular mechanisms, esterase expression could represent activation of proteases at the onset of spore germination. (C) 2004 Elsevier Inc. All rights reserved.