Evolved Differences in cis and trans Regulation Between the Maternal and Zygotic mRNA Complements in the Drosophila Embryo.
Evolved Differences in cis and trans Regulation Between the Maternal and Zygotic mRNA Complements in the Drosophila Embryo.
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DOI：
10.1534/genetics.120.303626
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发表时间：
2020-11
期刊：
影响因子：
3.3
通讯作者：
Lott SE
中科院分区：
文献类型：
--
作者：
Cartwright EL;Lott SE
早期发育的关键过程需要母亲在合子基因组转录激活之前将基因产物储存到卵子中。在这里，卡特赖特和洛特确定了转录本不同的基因调控进化模式。基因表达如何进化取决于驱动基因表达的机制。基因的表达在不同的发育阶段以不同的方式受到控制；在这里，我们问不同的发育阶段是否表现出不同的调控进化模式。为了探索调控进化的模式，我们使用了由两个不同基因组控制的胚胎发育的早期阶段，母亲的基因组和受精卵的基因组。在所有动物的胚胎发育过程中，最初的发育过程完全由母体提供的基因产物沉积到卵母细胞中。在母体向合子的转变过程中，当发育控制权从母体基因产物移交给合子时，合子基因组随后被激活。利用果蝇姊妹种(拟果蝇、拟果蝇和毛果果蝇)的杂交和转录组学，我们发现母体转录沉积的调节和合子转录的进化通过不同的机制。我们发现，相对于亲本物种，杂交后代的转录水平遗传模式在母本转录本和合子转录本之间有所不同，并且母本转录本水平更有可能是保守的。转录水平的变化主要是通过母体基因反式调节的差异发生的，而合子转录的变化是顺式和反式调节变化的共同作用。母体和受精卵潜在调控环境的差异可能是母体和受精卵转录本如何进化的主要决定因素。
The critical processes of early development require gene products that mothers deposit into eggs, before the zygotic genome is transcriptionally activated. Here, Cartwright and Lott determine that patterns of gene regulatory evolution differ for transcripts ..... How gene expression can evolve depends on the mechanisms driving gene expression. Gene expression is controlled in different ways in different developmental stages; here we ask whether different developmental stages show different patterns of regulatory evolution. To explore the mode of regulatory evolution, we used the early stages of embryonic development controlled by two different genomes, that of the mother and that of the zygote. During embryogenesis in all animals, initial developmental processes are driven entirely by maternally provided gene products deposited into the oocyte. The zygotic genome is activated later, when developmental control is handed off from maternal gene products to the zygote during the maternal-to-zygotic transition. Using hybrid crosses between sister species of Drosophila (D. simulans, D. sechellia, and D. mauritiana) and transcriptomics, we find that the regulation of maternal transcript deposition and zygotic transcription evolve through different mechanisms. We find that patterns of transcript level inheritance in hybrids, relative to parental species, differ between maternal and zygotic transcripts, and maternal transcript levels are more likely to be conserved. Changes in transcript levels occur predominantly through differences in trans regulation for maternal genes, while changes in zygotic transcription occur through a combination of both cis and trans regulatory changes. Differences in the underlying regulatory landscape in the mother and the zygote are likely the primary determinants for how maternal and zygotic transcripts evolve.