Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine
Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine
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DOI：
10.1016/j.biortech.2008.10.057
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发表时间：
2009-04-01
影响因子：
11.4
通讯作者：
Gleisner, R.
中科院分区：
文献类型：
--
作者：
Zhu, J. Y.;Pan, X. J.;Gleisner, R.
这项研究建立了一种使用亚硫酸盐预处理来克服木质纤维素 (SPORL) 顽固性的新工艺，从而实现软木的稳健和高效的生物转化。该工艺包括在酸性条件下对木片进行亚硫酸盐处理，然后使用盘磨机进行机械尺寸减小。结果表明，用 8-10% 亚硫酸氢盐和 1.8-3.7% 硫酸对云杉片进行 SPORL 预处理，对烘干 (od) 木材在 180 ℃ 下处理 30 分钟，水解 48 小时后，底物的纤维素转化率达到 90% 以上，酶负载量为每粒 od 底物约 14.6 FPU 纤维素酶和 22.5 CBU β-葡萄糖苷酶。每 100 克未经处理的 od 云杉木（葡聚糖含量 43%）酶水解底物产生的葡萄糖产量约为 37 克（不包括预处理过程中溶解的葡萄糖）。研究发现，在 SPORL 工艺中，半纤维素的去除对于纤维素转化来说与木质素磺化一样重要。预处理改变了木片，从而将尺寸减小所需的电能消耗降低至约 19 Wh/kg od 未经处理的木材。或约 19 克葡萄糖/瓦时电量。此外，SPORL 产生少量的发酵抑制剂羟甲基糠醛 (HMF) 和糠醛，分别为每克未处理的木材约 5 毫克和 1 毫克。此外，当 SPORL 应用于红松时，也取得了类似的结果。通过建立在制浆造纸行业已经实践的成熟亚硫酸盐制浆和盘式精炼技术的基础上，SPORL 的商业化技术障碍和风险非常少。由爱思唯尔有限公司出版
This study established a novel process using sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) for robust and efficient bioconversion of softwoods. The process consists of sulfite treatment of wood chips under acidic conditions followed by mechanical size reduction using disk refining. The results indicated that after the SPORL pretreatment of spruce chips with 8-10% bisulfite and 1.8-3.7% sulfuric acid on oven dry (od) wood at 180 degrees C for 30 min, more than 90% cellulose conversion of substrate was achieved with enzyme loading of about 14.6 FPU cellulase plus 22.5 CBU beta-glucosidase per grain of od substrate after 48 h hydrolysis. Glucose yield from enzymatic hydrolysis of the substrate per 100 g of untreated od spruce wood (glucan content 43%) was about 37 g (excluding the dissolved glucose during pretreatment). Hemicellulose removal was found to be as critical as lignin sulfonation for cellulose conversion in the SPORL process. Pretreatment altered the wood chips, which reduced electric energy consumption for size reduction to about 19 Wh/kg od untreated wood. or about 19 g glucose/Wh electricity. Furthermore, the SPORL produced low amounts of fermentation inhibitors, hydroxymethyl furfural (HMF) and furfural, of about 5 and 1 mg/g of untreated od wood, respectively. In addition, similar results were achieved when the SPORL was applied to red pine. By building on the mature sulfite pulping and disk refining technologies already practiced in the pulp and paper industry, the SPORL has very few technological barriers and risks for commercialization. Published by Elsevier Ltd.