Differentially Expressed miRNAs in Tumor, Adjacent, and Normal Tissues of Lung Adenocarcinoma
Differentially Expressed miRNAs in Tumor, Adjacent, and Normal Tissues of Lung Adenocarcinoma
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肺腺癌肿瘤组织、癌旁组织和正常组织中差异表达的 miRNA
DOI：
10.1155/2016/1428271
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发表时间：
2016-01-01
影响因子：
--
通讯作者：
Ge, Qinyu
中科院分区：
文献类型：
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作者：
Tian, Fei;Li, Rui;Ge, Qinyu
肺癌是癌症死亡的主要原因。非小细胞肺癌（NSCLC）是肺癌的主要类型。本研究的目的是描述腺癌（AC）中miRNA的表达谱，AC是NSCLC的主要亚型之一。在这项研究中，通过下一代测序在正常组织、癌旁组织和肿瘤组织中检测到miRNAs。然后通过定量逆转录-聚合酶链反应（qRT-PCR）定量差异miRNA的表达水平。在结果中，在合并的AC样品的肿瘤、邻近和正常组织中分别检测到259、401和389个miRNA。此外，我们首次发现miR-21- 5 p和miR-196 a-5 p从正常组织到癌旁组织逐渐上调; miR-218- 5 p在AC组织中逐渐下调，变化为2倍或更大。通过qRT-PCR验证这3种miRNA。最后，对这3种miRNAs的靶基因进行了预测，丰富了其潜在的功能和调控途径。miR-21- 5 p、miR-196 a-5 p和miR-218- 5 p可能成为肺腺癌诊断和预后的生物标志物。本研究对肺腺癌的诊断及肺癌的病理学研究有一定的参考价值。
Lung cancer is the leading cause of cancer deaths. Non-small-cell lung cancer (NSCLC) is the major type of lung cancer. The aim of this study was to characterize the expression profiles of miRNAs in adenocarcinoma (AC), one major subtype of NSCLC. In this study, the miRNAs were detected in normal, adjacent, and tumor tissues by next-generation sequencing. Then the expression levels of differential miRNAs were quantified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). In the results, 259, 401, and 389 miRNAs were detected in tumor, adjacent, and normal tissues of pooled AC samples, respectively. In addition, for the first time we have found that miR-21-5p and miR-196a-5p were gradually upregulated from normal to adjacent to tumor tissues; miR-218-5p was gradually downregulated with 2-fold or greater change in AC tissues. These 3 miRNAs were validated by qRT-PCR. Lastly, we predicted target genes of these 3 miRNAs and enriched the potential functions and regulatory pathways. The aberrant miR-21-5p, miR-196a-5p, and miR-218-5p may become biomarkers for diagnosis and prognosis of lung adenocarcinoma. This research may be useful for lung adenocarcinoma diagnosis and the study of pathology in lung cancer.