Long lasting immunity in chickens induced by a single shot of influenza vaccine prepared from inactivated non-pathogenic H5N1 virus particles against challenge with a highly pathogenic avian influenza virus
Long lasting immunity in chickens induced by a single shot of influenza vaccine prepared from inactivated non-pathogenic H5N1 virus particles against challenge with a highly pathogenic avian influenza virus
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DOI：
10.1016/j.vaccine.2009.06.070
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发表时间：
2009-08-20
期刊：
影响因子：
5.5
通讯作者：
Kida, Hiroshi
中科院分区：
文献类型：
--
作者：
Sasaki, Takashi;Kokumai, Norihide;Kida, Hiroshi
使用含有脱水甘露醇-八烯酸酯-醚（AMOE）的油佐剂，从非致病性株A/鸭/北海道/Vac-1/04（H5 N1）病毒的灭活全颗粒制备流感疫苗。将该疫苗肌肉注射到5只4周龄鸡中，并在接种后138周，用100倍50%鸡致死量的高致病性禽流感（HPAI）病毒A/chicken/Yamaguchi/7/04（H5 N1）鼻内攻击它们。所有5只鸡均存活，未表现出流感的临床体征，但攻毒后2天，3只接种鸡在咽喉拭子中脱落了有限滴度的病毒。(C)2009爱思唯尔有限公司保留所有权利。
An influenza vaccine was prepared from inactivated whole particles of the non-pathogenic strain A/duck/Hokkaido/Vac-1/04 (H5N1) virus using an oil adjuvant containing anhydromannitol-octaclecenoate-ether (AMOE). The vaccine was injected intramuscularly into five 4-week-old chickens, and 138 weeks after vaccination, they were challenged intranasally with 100 times 50% chicken lethal dose of the highly pathogenic avian influenza (HPAI) virus A/chicken/Yamaguchi/7/04 (H5N1). All 5 chickens survived without exhibiting clinical signs of influenza, although 2 days post-challenge, 3 vaccinated chickens shed limited titres of viruses in laryngopharyngeal swabs. (C) 2009 Elsevier Ltd. All rights reserved.