Infrared Spectra of Microtome Sections of Human Colon Tissues
Infrared Spectra of Microtome Sections of Human Colon Tissues
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人类结肠组织切片的红外光谱
DOI：
10.1366/0003702904417481
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发表时间：
1990
影响因子：
3.5
通讯作者：
B. Rigas
中科院分区：
文献类型：
--
作者：
P. Wong;B. Rigas
在我们的渥太华实验室中开发的用于生物组织的压力调谐红外光谱技术使得使用傅里叶变换红外光谱用于细胞和组织切片的研究成为可能。最近，使用这种技术，我们研究了人类结肠癌组织标本中生物分子的结构特性。在这项工作中，我们发现，恶性结肠组织可以区分正常结肠组织的一系列高压红外光谱参数。此外，我们观察到，在大气压力下获得的一些红外参数的恶性结肠组织与正常结肠组织有显着差异。例如，在正常和恶性结肠组织之间，核酸的对称磷酸盐伸缩带的频率存在2- 3cm-1的差异。
The development of a pressure-tuned infrared spectroscopic technique for biological tissues in our Ottawa laboratory has made possible the use of Fourier transform infrared spectroscopy for the study of cells and tissue sections. Recently, using this technique we investigated structural properties of biomolecules in tissue specimens of human colon cancer. During this work we discovered that malignant colon tissue can be distinguished from normal colon tissue by a series of high-pressure infrared spectroscopic parameters. Furthermore, we observed that some infrared parameters of malignant colonic tissue obtained at atmospheric pressure were significantly different from those of normal colonic tissue. For example, there is a 2–3 cm−1 difference in the frequency of the symmetric phosphate stretching band of the nucleic acids between normal and malignant colon tissues.