Molecular and cytogenetic organization of the 5S ribosomal DNA array in chicken (Gallus gallus)
Molecular and cytogenetic organization of the 5S ribosomal DNA array in chicken (Gallus gallus)
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DOI：
10.1023/a:1024008522122
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发表时间：
2003-04-01
影响因子：
2.6
通讯作者：
Delany, ME
中科院分区：
文献类型：
--
作者：
Daniels, LM;Delany, ME
5S核糖体（r）RNA基因编码核糖体大亚基的高度保守的小组分（类似于120-bp）。本研究的目的是研究鸡5S rDNA的分子和细胞遗传学组织。在10个研究和商业群体中鉴定出一个由编码区和基因间间隔区（IGS）组成的2.2kb的优势基因（5Salpha）。在一些人群中观察到0.6 kb（5Sbeta）的变异基因重复。遗传连锁分析和荧光原位杂交细胞遗传学定位分配的5S rDNA的9号染色体。在EcoRV消化后进行电泳大小测定时，5S rDNA阵列被确定为80.2+/-7.0 kb。5 SalphaIGS区域的序列分析显示相当大的保守性鸡亚种之间（98.4%的同一性），以及与脊椎动物的Pol III启动子和调控序列基序的同源性。在4个克隆的红原鸡（Gallus gallus gallus）5 Salpha重复序列中，在IGS的1000 bp内观察到了微小的个体内序列变异（95.5%的同一性）。5Salpha和5Sbeta基因的IGS区序列比较表明，两个短的连续（>20 bp）和许多短的非连续同源区以及其他保守特征，如启动子和终止基序。
The 5S ribosomal (r) RNA genes encode a small (similar to120-bp) highly-conserved component of the large ribosomal subunit. The objective of the present research was to study the molecular and cytogenetic organization of the chicken 5S rDNA. A predominant 2.2-kb gene (5Salpha) consisting of a coding and intergenic spacer (IGS) region was identified in ten research and commercial populations. A variant gene repeat of 0.6 kb (5Sbeta) was observed in some of the populations. Genetic linkage analysis and cytogenetic localization by fluorescence in-situ hybridization assigned the 5S rDNA to chromosome 9. The 5S rDNA array was determined to be 80.2+/-7.0 kb upon electrophoretic sizing following EcoRV digestion. Sequence analysis of 5Salpha IGS regions revealed considerable conservation between chicken subspecies (98.4% identity) as well as homology with vertebrate Pol III promoter and regulatory sequence motifs. Minor intraindividual sequence variation within 1000 bp of IGS was observed in four cloned Red Jungle Fowl (Gallus gallus gallus) 5Salpha repeats (95.5% identity in this region). Sequence comparisons between IGS regions of 5Salpha and 5Sbeta genes indicated two short continuous (>20 bp) and many short non-continuous homologous regions as well as other conserved features such as promoter and termination motifs.