Global gene expression analysis reveals specific and redundant roles for H1 variants, H1c and H10, in gene expression regulation
Global gene expression analysis reveals specific and redundant roles for H1 variants, H1c and H10, in gene expression regulation
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DOI：
10.1016/j.gene.2008.01.025
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发表时间：
2008-05-15
期刊：
影响因子：
3.5
通讯作者：
Sittman, Donald B.
中科院分区：
文献类型：
--
作者：
Bhan, Sheetal;May, Warren;Sittman, Donald B.
在哺乳动物中，存在进化上保守的多个非等位基因H1变体的功能意义仍不清楚。我们使用了一种独特的过度生产方法，结合细胞周期同步和早期时间点测定，以评估H1变体H1c和H1（0）在不存在可能掩盖变体特异性效应的补偿事件的情况下对全局基因表达的差异效应。我们发现H1c和H10主要作为基因表达的特异性而非全局调节因子。许多受影响的基因都是H1c或H10的独特靶向基因，这证实了H1变体具有一些独特的作用。我们还确定了受这两种变体影响的基因，在这种情况下，这些基因的表达在很大程度上受到这两种变体的影响。这一观察结果表明，除了具有特定的功能外，H1变体在染色质的组织中具有共同的作用。我们进一步注意到，H1（0）比H1c抑制更多的基因，这可能是H1（0）是一个更强的转录抑制因子的流行观点的基础。(c)2008 Elsevier B.V保留所有权利。
In mammals, the functional significance of the presence of evolutionarily conserved, multiple non-allelic H1 variants remains unclear. We used a unique overproduction approach coupled with cell cycle synchronization and early time point assays to assess differential effects of H1 variants, H1c and H1(0), on global gene expression in the absence of compensatory events that may mask variant-specific effects. We found that H1c and H 10 act primarily as specific rather than global regulators of gene expression. Many of the genes affected were uniquely targeted by either H1c or H 10, affirming that H1 variants have some unique roles. We also identified genes that were affected by both variants, in which cases the expression of these genes was, for the most part, affected similarly by both the variants. This observation suggests that as well as having specific functions, the H1 variants share common roles in the organization of chromatin. We further noted that H1(0) repressed more genes than did H1c, which may underlie the prevailing notion that H1(0) is a stronger repressor of transcription. (c) 2008 Elsevier B.V All rights reserved.