Biosynthesis of Au, Ag and Au-Ag bimetallic nanoparticles using protein extracts of Deinococcus radiodurans and evaluation of their cytotoxicity.
Biosynthesis of Au, Ag and Au-Ag bimetallic nanoparticles using protein extracts of Deinococcus radiodurans and evaluation of their cytotoxicity.
复制标题
使用耐辐射奇球菌蛋白提取物生物合成金、银和金银双金属纳米粒子及其细胞毒性评价
DOI：
10.2147/ijn.s149079
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发表时间：
2018
影响因子：
8
通讯作者：
Hua Y
中科院分区：
文献类型：
--
作者：
Li J;Tian B;Li T;Dai S;Weng Y;Lu J;Xu X;Jin Y;Pang R;Hua Y
贵金属纳米粒子具有环境友好和生物相容性，因此其生物合成方法受到了广泛的关注。在这项研究中，我们研究了Au，Ag和Au-Ag纳米粒子的合成使用的蛋白质提取物的耐辐射，表现出强大的金属还原能力。通过各种光谱技术表征和分析所获得的NP。地方与D.抗辐射蛋白提取物介导的金纳米颗粒（Drp-AuNPs）。Drp-AgNPs和Drp-AuNPs表现出球形形态，在pH 7时，平均尺寸分别为37.13±5.97 nm和51.72±7.38 nm，zeta电位值分别为−18.31±1.39 mV和−15.17±1.24 mV。在24 h时测得的Drp-AuNPs和Drp-AgNPs的释放效率分别为3.99%和18.20%。在合成过程中，Au（III）被还原为Au（I），并进一步还原为Au（0），Ag（I）通过与蛋白质的羟基、胺、羧基、磷酸基或巯基相互作用被还原为Ag（0），随后被这些基团稳定。Drp-AuNPs与Drp-AgNPs的一些特性不同，这可能是由于它们与不同的蛋白质结合基团相互作用的结果。Drp-AgNPs可以通过电置换反应进一步形成Au-Ag纳米粒子。Drp-AuNPs和Au-AgNPs对MCF-10A细胞的细胞毒性较低，这是由于细胞内活性氧（ROS）的产生水平低于Drp-AgNPs。这些结果对于理解利用细菌蛋白质提取物生物合成贵金属纳米颗粒的机制和性质至关重要。生物相容性Au或Au-Ag纳米粒子可应用于生物传感、生物成像和生物医学。
Biosynthesis of noble metallic nanoparticles (NPs) has attracted significant interest due to their environmental friendly and biocompatible properties. In this study, we investigated syntheses of Au, Ag and Au–Ag bimetallic NPs using protein extracts of Deinococcus radiodurans, which demonstrated powerful metal-reducing ability. The obtained NPs were characterized and analyzed by various spectroscopy techniques. The D. radiodurans protein extract-mediated silver nanoparticles (Drp-AgNPs) were preferably monodispersed and stably distributed compared to D. radiodurans protein extract-mediated gold nanoparticles (Drp-AuNPs). Drp-AgNPs and Drp-AuNPs exhibited spherical morphology with average sizes of 37.13±5.97 nm and 51.72±7.38 nm and zeta potential values of −18.31±1.39 mV and −15.17±1.24 mV at pH 7, respectively. The release efficiencies of Drp-AuNPs and Drp-AgNPs measured at 24 h were 3.99% and 18.20%, respectively. During the synthesis process, Au(III) was reduced to Au(I) and further to Au(0) and Ag(I) was reduced to Ag(0) by interactions with the hydroxyl, amine, carboxyl, phospho or sulfhydryl groups of proteins and subsequently stabilized by these groups. Some characteristics of Drp-AuNPs were different from those of Drp-AgNPs, which could be attributed to the interaction of the NPs with different binding groups of proteins. The Drp-AgNPs could be further formed into Au–Ag bimetallic NPs via galvanic replacement reaction. Drp-AuNPs and Au–Ag bimetallic NPs showed low cytotoxicity against MCF-10A cells due to the lower level of intracellular reactive oxygen species (ROS) generation than that of Drp-AgNPs. These results are crucial to understand the biosynthetic mechanism and properties of noble metallic NPs using the protein extracts of bacteria. The biocompatible Au or Au–Ag bimetallic NPs are applicable in biosensing, bioimaging and biomedicine.