Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells
Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells
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DOI：
10.1038/nn.2473
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发表时间：
2010-02-01
影响因子：
25
通讯作者：
Nakamura, Shun
中科院分区：
文献类型：
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作者：
Ohira, Koji;Furuta, Takahiro;Nakamura, Shun
成年哺乳动物的神经发生发生在海马体和嗅球中，而新皮质成人神经发生仍然存在争议。最近报道了在受伤的新皮质中发生的几起新皮质成人神经发生，这表明可以通过损伤激活的神经干细胞（NSC）或神经元祖细胞（NPC）保留在成人大脑中。然而，尚不清楚大脑中是否存在新皮质 NSC/NPC，以及在何处存在。我们在成年大鼠的新皮质第一层中发现了 NPC，并观察到它们的增殖因整体前脑缺血而高度激活。使用逆转录病毒介导的膜靶向绿色荧光蛋白标记第 1 层增殖细胞，我们发现新生成的神经元具有 GABA 能，并且神经元在功能上整合到神经元回路中。我们的结果表明，第 1 层 NPC 是缺血条件下成人神经发生的来源。
Adult mammalian neurogenesis occurs in the hippocampus and the olfactory bulb, whereas neocortical adult neurogenesis remains controversial. Several occurrences of neocortical adult neurogenesis in injured neocortex were recently reported, suggesting that neural stem cells (NSCs) or neuronal progenitor cells (NPCs) that can be activated by injury are maintained in the adult brain. However, it is not clear whether or where neocortical NSCs/NPCs exist in the brain. We found NPCs in the neocortical layer 1 of adult rats and observed that their proliferation was highly activated by global forebrain ischemia. Using retrovirus-mediated labeling of layer 1 proliferating cells with membrane-targeted green fluorescent protein, we found that the newly generated neurons were GABAergic and that the neurons were functionally integrated into the neuronal circuitry. Our results suggest that layer 1 NPCs are a source of adult neurogenesis under ischemic conditions.