THE COMPLETE NUCLEOTIDE-SEQUENCE OF SWINE VESICULAR DISEASE VIRUS
THE COMPLETE NUCLEOTIDE-SEQUENCE OF SWINE VESICULAR DISEASE VIRUS
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DOI：
10.1099/0022-1317-70-4-919
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发表时间：
1989-04-01
影响因子：
3.8
通讯作者：
SEKIGUCHI, K
中科院分区：
文献类型：
--
作者：
INOUE, T;SUZUKI, T;SEKIGUCHI, K
使用分子克隆和 DNA 测序技术确定了从健康猪中分离的肠道病毒猪水泡病病毒（SVDV；H/3''76）基因组的完整核苷酸序列。 RNA基因组长7400个核苷酸，不包括poly(A)束，并且似乎编码2185个氨基酸的单一多蛋白。预测的多蛋白氨基酸序列与先前测序的柯萨奇病毒B1、B3和B4的氨基酸序列显示出密切的同源性（约90%），并且与脊髓灰质炎病毒的氨基酸序列也显示出同源性（约60%）。这种同源性使我们能够预测多蛋白可能的切割位点，并识别具有结构和功能意义的其他特征，这似乎对病毒的生物完整性很重要。对 SVDV 和柯萨奇病毒之间同源性的详细分析表明，非结构蛋白高度保守，而结构蛋白则不太保守。 5''和3''非编码区也是保守的，尽管有几个不同的核苷酸片段。这些延伸可以将 SVDV 与柯萨奇病毒区分开来。
The complete nucleotide sequence of the genome of the enterovirus swine vesicular disease virus (SVDV; H/3 ''76) isolated from a healthy pig has been determined using molecular cloning and DNA sequencing techniques. The RNA genome was 7400 nucleotides long, excluding the poly(A) tract, and appeared to encode a single polyprotein of 2185 amino acids. The predicted amino acid sequence of the polyprotein showed close homology (around 90%) to that of the previously sequenced coxsackieviruses B1, B3 and B4, and also showed homology (around 60%) to that of poliovirus. This homology allows us to predict the possible cleavage sites of the polyprotein and to identify other features of structural and functional significance, which seem to be important to the biological integrity of the virus. A detailed analysis of homology between SVDV and coxsackieviruses shows that non-structural proteins are highly conserved whereas the structural proteins are less well conserved. the 5'' and 3'' noncoding regions are also conserved, although there are several divergent nucleotide stretches. These stretches may differentiate SVDV from coxsackieviruses.