Vear, a Novel Golgi-associated Protein with VHS and γ-Adaptin “Ear” Domains*
Vear, a Novel Golgi-associated Protein with VHS and γ-Adaptin “Ear” Domains*
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Vear，一种具有 VHS 和 γ-Adaptin“Ear”结构域的新型高尔基体相关蛋白*
DOI：
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发表时间：
2000
影响因子：
4.8
通讯作者：
V. Lehto
中科院分区：
文献类型：
--
作者：
A. Poussu;O. Lohi;V. Lehto
内吞和分泌途径中囊泡形成的选择性和细节的分子基础仍然知之甚少，最有可能涉及尚未鉴定的组分。在这里，我们描述了一种新的67 kDa的蛋白质（称为Vear）的克隆，表达，组织和细胞分布，它具有同源性的几个内吞相关蛋白质，在它的N末端有一个VHS结构域。它也与AP-1的重亚基γ-adaptin相似，在其C末端具有典型的“耳”结构域。在免疫荧光显微镜下，通过典型的分布模式、与高尔基体标志物γ-适应素的明显共定位以及对布雷菲德菌素A处理细胞的敏感性判断，在高尔基体复合体中观察到Vear。在细胞分级分离中，维尔与后核膜部分一起分配。在转染实验中，血凝素标记的全长Vear和截短的Vear缺乏VHS结构域组装在高尔基体复合体上并引起高尔基体复合体的压缩。高尔基体协会没有压实被视为与耳域的Vear，而VHS域单独表现出弥漫性膜和囊泡相关的分布。高尔基体协会和二分结构沿着与其结构域的差异靶向表明，Vear参与异型囊泡/亚细胞器的相互作用与高尔基体复合体。Vear在肾脏、肌肉和心脏中的高水平表达表明其具有组织特异性功能。
The molecular basis of the selectivity and the details of the vesicle formation in endocytic and secretory pathways are still poorly known and most probably involve as yet unidentified components. Here we describe the cloning, expression, and tissue and cell distribution of a novel protein of 67 kDa (called Vear) that bears homology to several endocytosis-associated proteins in that it has a VHS domain in its N terminus. It is also similar to γ-adaptin, the heavy subunit of AP-1, in having in its C terminus a typical “ear” domain. In immunofluorescence microscopy, Vear was seen in the Golgi complex as judged by a typical distribution pattern, a distinct colocalization with the Golgi marker γ-adaptin, and a sensitivity to treatment of cells with brefeldin A. In cell fractionation, Vear partitioned with the post-nuclear membrane fraction. In transfection experiments, hemagglutinin-tagged full-length Vear and truncated Vear lacking the VHS domain assembled on and caused compaction of the Golgi complex. Golgi association without compaction was seen with the ear domain of Vear, whereas the VHS domain alone showed a diffuse membrane- and vesicle-associated distribution. The Golgi association and the bipartite structure along with the differential targeting of its domains suggest that Vear is involved in heterotypic vesicle/suborganelle interactions associated with the Golgi complex. Tissue-specific function of Vear is suggested by its high level of expression in kidney, muscle, and heart.
一种膜相关蛋白复合物，选择性结合网格蛋白外壳接头 AP1。
影响因子：
4
作者：
Mallet,WG;Brodsky,FM
通讯作者：
Brodsky,FM