Gelatin Templated Polypeptide Co-Cross-Linked Hydrogel for Bone Regeneration
Gelatin Templated Polypeptide Co-Cross-Linked Hydrogel for Bone Regeneration
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用于骨再生的明胶模板多肽共交联水凝胶
DOI：
10.1002/adhm.201901239
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发表时间：
2019-12-09
影响因子：
10
通讯作者：
Shi, Qin
中科院分区：
文献类型：
--
作者：
Qiao, Yusen;Liu, Xingzhi;Shi, Qin
氨基酸短链多肽具有多种生物学功能，在临床上得到了广泛的应用。然而，这些多肽的易失活特性和突释特性限制了它们在体内的应用。在这里，一种新的成骨多肽水凝胶（GelMA-c-OGP）是通过共交联模板光交联明胶（GelMA）与光交联成骨生长肽（OGP）使用紫外线辐射。GelMA能够形成具有良好机械性能的具有光交叉交联OGP的水凝胶，并且还促进骨再生。GelMA-c-OGP水凝胶通过显著增强成骨相关基因BMP-2、OCN和OPN的表达，并增加成骨细胞中钙盐的沉淀，加速成骨前体细胞的成骨过程。类似地，GelMA-c-OGP水凝胶促进体内骨再生。此外，通过苏木精-伊红和I型胶原和TGF-β的免疫组织化学染色，观察到GelMA-c-OGP植入组中比对照组有更多的胶原纤维连接皮质骨。共交联的OGP多肽在瞬时紫外光的原位作用下从液体转化为固体水凝胶，还可以增强缺损骨的力学性能，避免成骨肽在骨缺损愈合期间破裂释放。总的来说，与传统方法相比，这种水凝胶递送系统对骨缺损愈合具有显著影响。
Polypeptides with short chains of amino acid monomers have been widely applied in the clinic because of their various biological functions. However, the easily-inactivated characteristics and burst releasing of the peptides limit their application in vivo. Here, a novel osteogenic polypeptide hydrogel (GelMA-c-OGP) is created by co-cross-linking template photo-cross-linked gelatin (GelMA) with photo-cross-linkable osteogenic growth peptides (OGP) using ultraviolet radiation. GelMA enables the formation of hydrogel with photo-cross-linkable OGP with good mechanical properties and also promotes bone regeneration. GelMA-c-OGP hydrogel accelerates the bone formation procedure of osteogenic precursor cells by significantly enhancing the expression of osteogenic-related genes BMP-2, OCN, and OPN, and increasing the precipitation of calcium salts in osteoblasts. Similarly, GelMA-c-OGP hydrogel promotes bone regeneration in vivo. Furthermore, it is observed that more collagen fibers connect cortical bones in the GelMA-c-OGP implanted group than the control group by hematoxylin-eosin and immunohistochemical staining of Collagen I and TGF-beta. The co-cross-linked OGP polypeptide converts from liquid to solid hydrogel with transient UV light in situ, which also can strengthen the mechanical property of the defect bone and avoid burst osteogenic peptide, releasing during the bone defect healing period. Overall, this hydrogel delivering system has a significant impact on bone defect healing compared with traditional methods.