Sorting nexins 1 and 2a locate mainly to the TGN
Sorting nexins 1 and 2a locate mainly to the TGN
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DOI：
10.1007/s00709-012-0399-1
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发表时间：
2013-02-01
期刊：
影响因子：
2.9
通讯作者：
Robinson, David G.
中科院分区：
文献类型：
--
作者：
Stierhof, York-Dieter;Viotti, Corrado;Robinson, David G.
高等植物中分选连接蛋白 (SNX) 的亚细胞定位存在争议。先前对表达 SNX1-GFP 的转基因拟南芥系根细胞进行的共聚焦激光扫描显微镜（CLSM 研究）表明，该 SNX 存在于具有反式高尔基体网络 (TGN) 和多泡体 (MVB) 特征的内体上。相反，当在烟草叶肉原生质体中瞬时表达时，SNX2a 仅定位于 TGN。通过对冷冻固定的拟南芥根进行免疫金电子显微镜，我们发现试图澄清这种情况。SNX1-GFP 和内源性 SNX2a 主要定位于 TGN，但无法确定。
The subcellular localization of the sorting nexins (SNXs) in higher plants is a matter of controversy. Previous confocal laser scanning microscopy (CLSM studies on root cells from a transgenic Arabidopsis line expressing SNX1-GFP have suggested that this SNX is present on an endosome having characteristics of both the trans-Golgi network (TGN) and the multivesicular body (MVB). In contrast, SNX2a locates exclusively to the TGN when transiently expressed in tobacco mesophyll protoplasts. By performing immunogold electron microscopy on cryofixed Arabidopsis roots, we have tried to clarify the situation. Both SNX1-GFP and endogenous SNX2a locate principally to the TGN. Labeling of MVBs could not be confirmed with any certainty.