Single-Cell Tracking of Breast Cancer Cells Enables Prediction of Sphere Formation from Early Cell Divisions.
Single-Cell Tracking of Breast Cancer Cells Enables Prediction of Sphere Formation from Early Cell Divisions.
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DOI：
10.1016/j.isci.2018.08.015
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发表时间：
2018-10-26
期刊：
影响因子：
5.8
通讯作者：
Martin SS
中科院分区：
文献类型：
--
作者：
Bailey PC;Lee RM;Vitolo MI;Pratt SJP;Ory E;Chakrabarti K;Lee CJ;Thompson KN;Martin SS
乳房试验已被广泛用于量化人群中的干细胞样细胞。然而，问题是现场并没有采用标准的协议。据报道，细胞接种密度为1,000-100,000个/毫升。这些高密度导致细胞聚集。为了解决这个问题，我们在14天的时间里分别跟踪了1,127个单一的MCF-7和696个单一的T47D人类乳腺肿瘤细胞。此跟踪为我们提供了5天、7天和14天的常用终端的详细信息，这些终端不受蜂窝聚合的影响。这包括平均球体大小，球体形成效率，以及两条线的定义明确的最小尺寸。重要的是，我们已经将早期的细胞分裂与最终的球体形成联系起来。在播种后24小时，我们可以预测第14天的球体总数，两条线都有98%的准确率。这种方法消除了细胞聚集，并有可能将5-14天的检测缩短到24小时。单细胞跟踪去除乳房大气层中混杂的聚集分析跟踪显示球体形成效率远高于通常报道的球体真正的克隆球体在24小时内比通常报道的要小，跟踪可以用98%的准确性预测总共14天的球体生物学实验方法；癌症
The mammosphere assay has become widely employed to quantify stem-like cells in a population. However, the problem is there is no standard protocol employed by the field. Cell seeding densities of 1,000 to 100,000 cells/mL have been reported. These high densities lead to cellular aggregation. To address this, we have individually tracked 1,127 single MCF-7 and 696 single T47D human breast tumor cells by eye over the course of 14 days. This tracking has given us detailed information for the commonly used endpoints of 5, 7, and 14 days that is unclouded by cellular aggregation. This includes mean sphere sizes, sphere-forming efficiencies, and a well-defined minimum size for both lines. Importantly, we have correlated early cell division with eventual sphere formation. At 24 hr post seeding, we can predict the total spheres on day 14 with 98% accuracy in both lines. This approach removes cell aggregation and potentially shortens a 5- to 14-day assay to a 24 hours. Single-cell tracking removes confounding aggregation from the mammosphere assay Tracking reveals sphere-forming efficiencies much higher than commonly reported True clonal spheres are smaller than commonly reported At 24 hours, tracking can predict total day 14 spheres with 98% accuracy Biology Experimental Methods; Cancer
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影响因子：
10.5
作者：
Dontu, G;Abdallah, WM;Wicha, MS
通讯作者：
Wicha, MS
影响因子：
3.8
作者：
Gu, Wenyi;Prasadam, Indira;Yu, Chengzhong
通讯作者：
Yu, Chengzhong
DOI：
10.1186/bcr2106
发表时间：
2008
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Grimshaw MJ;Cooper L;Papazisis K;Coleman JA;Bohnenkamp HR;Chiapero-Stanke L;Taylor-Papadimitriou J;Burchell JM
通讯作者：
Burchell JM
影响因子：
3.7
作者：
Morrison, Brian J.;Hastie, Marcus L.;Lopez, J. Alejandro
通讯作者：
Lopez, J. Alejandro
影响因子：
--
作者：
Niit, Maximilian;Hoskin, Victoria;Raptis, Leda
通讯作者：
Raptis, Leda