Two CBL genes from Populus euphratica confer multiple stress tolerance in transgenic triploid white poplar
Two CBL genes from Populus euphratica confer multiple stress tolerance in transgenic triploid white poplar
复制标题
胡杨的两个 CBL 基因赋予转基因三倍体白杨多重胁迫耐受性
DOI：
10.1007/s11240-011-0112-7
复制
发表时间：
2012-06-01
影响因子：
3
通讯作者：
Yin, Weilun
中科院分区：
文献类型：
--
作者：
Li, Dandan;Song, Shuyu;Yin, Weilun
在植物中，钙调神经磷酸酶B样蛋白（CBL）家族是一组独特的钙传感器，通过特异性地与CBL相互作用蛋白激酶家族相互作用并调节CBL相互作用蛋白激酶家族，在解码钙瞬变中发挥关键作用。本研究利用逆转录-聚合酶链反应（RT-PCR）技术从胡杨中克隆了两个CBL基因（PeCBL 6和PeCBL 10）。将花椰菜花叶病毒35 S启动子驱动的相应cDNA克隆到植物表达载体pCAMBIA 1301中，并导入三倍体白色毛白杨（Popolus tomentosa 'YixianCiZhu B385'）中，研究其功能。对转基因株系和野生型（WT）的叶片萎蔫率、高生长速率、叶绿素含量、丙二醛（MDA）含量和相对电导率（REC）等生理指标进行了测定和比较。结果表明，在低温胁迫下，转基因杨树的MDA含量和REC值均显著低于野生型杨树，在高盐、干旱和低温胁迫下，PeCBL基因的存在可以诱导杨树的高生长速率增加，枯萎叶片数减少。这些结果清楚地表明，转基因植物比非转基因幼苗具有更大的耐受性，这表明PeCBLs可能在胁迫耐受性中发挥重要作用。
In plants, the calcineurin B-like protein (CBL) family is a unique group of calcium sensors that play a key role in decoding calcium transients by specifically interacting with and regulating a family of CBL-interacting protein kinases. In this study, two CBL genes (PeCBL6 and PeCBL10) from Populus euphratica were isolated by reverse transcription-polymerase chain reaction. To examine their functions, the corresponding cDNAs, which expression was driven by the cauliflower mosaic virus 35S promoter, were cloned into a plant expression vector pCAMBIA1301 and introduced into the triploid white poplar (Popolus tomentosa ‘YiXianCiZhu B385’). The physiological parameters, including the percentage of wilted leaves, height growth rate, chlorophyll content, malondialdehyde (MDA) content and relative electrical conductivity (REC) of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that the MDA content and REC of transgenic plants were significantly lower in transgenic plants compared to WT plants when exposed to cold stress and that the presence of the PeCBL gene can induce an increase in height growth rate and a reduction in the number of wilted leaves in comparison to non-transgenic poplars under high salt, drought and cold stress. These results clearly demonstrate that transgenic plants had a greater tolerance to stresses than non-transgenic seedlings, suggesting that PeCBLs might play an important role in stress tolerance.