STABLE MAINTENANCE OF A 35-BASE-PAIR YEAST MITOCHONDRIAL GENOME
STABLE MAINTENANCE OF A 35-BASE-PAIR YEAST MITOCHONDRIAL GENOME
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DOI：
10.1128/mcb.9.5.1917
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发表时间：
1989-05-01
影响因子：
5.3
通讯作者：
BREWER, BJ
中科院分区：
文献类型：
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作者：
FANGMAN, WL;HENLY, JW;BREWER, BJ
分离并表征了源自野生型（[rho+]）酿酒酵母线粒体基因组的小缺失变体（[rho-]突变体）。检测的突变体线粒体DNA（mtDNAs）仅保留了一段基因间DNA的35个碱基对，完全由A. cntdot组成。T碱基对，并保持稳定。这些简单的mtDNA以串联重复阵列的形式存在，其扩增水平构成了. apprx。15%的总细胞DNA，并通过荧光显微镜判断，有一个几乎正常的线粒体排列在整个细胞质。这些[rho-]基因组的简单性质表明，维持mtDNA所需的序列必须非常简单。
Small deletion variants ([rho-] mutants) derived from the wild-type ([rho+]) Saccharomyces cerevisiae mitochondrial genome were isolated and characterized. The mutant mitochondrial DNAs (mtDNAs) examined retained as little as 35 base pairs of one section of intergenic DNA, were composed entirely of A .cntdot. T base pairs, and were stably maintained. These simple mtDNAs existed in tandemly repeated arrays at an amplified level that made up .apprx. 15% of the total cellular DNA and, as judged by fluorescence microscopy, had a nearly normal mitochondrial arrangement throughout the cell cytoplasm. The simple nature of these [rho-] genomes indicates that the sequences required to maintain mtDNA must be extremely simple.