Isolation and Functional Analysis of Human Neutrophils.
Isolation and Functional Analysis of Human Neutrophils.
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DOI：
10.1002/0471142735.im0723s111
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发表时间：
2015-11-02
影响因子：
--
通讯作者：
Zarember KA
中科院分区：
文献类型：
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作者：
Kuhns DB;Priel DAL;Chu J;Zarember KA
本单元描述了使用葡聚糖沉降和Percoll或Ficoll-Paque密度梯度从血液中分离人多形核中性粒细胞（PMN）。还描述了中性粒细胞功能的测定，包括呼吸爆发激活、吞噬作用和微生物杀灭。
This unit describes the isolation of human polymorphonuclear neutrophils (PMN) from blood using dextran sedimentation and Percoll or Ficoll-Paque density gradients. Assays of neutrophil functions including respiratory burst activation, phagocytosis, and microbial killing are also described.