Synthesis of cementum-like tissue in vitro by cells cultured from bone: a light and electron microscope study.
Synthesis of cementum-like tissue in vitro by cells cultured from bone: a light and electron microscope study.
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骨培养细胞在体外合成牙骨质样组织：光学和电子显微镜研究。
DOI：
10.1111/j.1600-0765.1986.tb01497.x
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发表时间：
1986
影响因子：
3.5
通讯作者：
A. Shiga
中科院分区：
文献类型：
--
作者：
A. H. Melcher;T. Cheong;J. Cox;E. Nemeth;A. Shiga
从胎鼠颅骨、人牙龈结缔组织和大鼠磨牙牙周韧带中获取细胞，分别与未脱钙和部分脱钙的根片在含有50μGG/ml抗坏血酸和10 mMβ-甘油磷酸的培养液中体外共培养，观察根片对细胞表型的影响。对培养物进行光镜和透射电子显微镜观察。在从大鼠颅骨获得的多层细胞中，观察到了类似于前人描述的骨样组织的结节。但在培养30天后，牙龈和牙周韧带细胞的多层细胞中不存在。与根片相关的头盖骨细胞产生的组织显示出类似于骨或细胞牙骨质的超微结构。体内无细胞牙骨质和无纤维牙骨质在相同时间后检查。在牙龈成纤维细胞和牙周膜细胞培养中未发现这些组织。这些观察表明，第一，来源于骨的细胞在体外可以表达牙骨质的表型；第二，来自人牙龈和大鼠牙周膜的细胞在相似的条件下培养时不能表达；第三，在牙周膜中发现的成骨细胞、成牙骨质细胞及其前体细胞可能至少部分地来自于它们的起源并从牙槽突的骨内间隙迁移到那里。
Cells obtained from calvariae of fetal rats, human gingival connective tissue and periodontal ligament of rat molars were each co-cultured in vitro with non-demineralized and partly demineralized root slices in nutrient medium containing 50 μGg/ml ascorbic acid and 10 mM B-glyeerophosphate, in order to determine whether the root slices could affect the phenotype expressed by the cells. The cultures were examined by light and transmission electron microscopy. Nodules of bone-like tissue, resembling those previously described by others, were observed in the multilayers of cells obtained from rat calvariae. but not in the multilayers of gingival and periodontal ligament cells, after 30 days of culture. Calvaria cells associated with the root slices produced tissues exhibiting ultrastructure that resembled that of bone or cellular cementum. acellular cemenlum and afibrillar cementum in vivo when examined after the same length of time. The tissues were never found in cultures of gingival fibroblasts or periodontal ligament cells. These observations suggest, first, that cells derived from bone can express in vitro the phenotype for the cementums; second, that cells obtained from human gingival and rat periodontal ligament do not do so when cultured under similar conditions; and third, the possibility that the osteoblasts, cementoblasts and their progenitors that are found in the periodontal ligament could, at least in part, have their origin and migrate there from the endosteal spaces of the alveolar process.